alpha-Actinin 1 Antibody (910301) [Unconjugated]

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat a-Actinin 1 Monoclonal Antibody (Catalog # MAB8279) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-Actinin 1 at approximately 100-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of C2C12 mouse myoblast cell line, loaded at 0.5 mg/mL. A specific band was detected for alpha -Actinin 1 at approximately 98 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human/Mouse/Rat alpha -Actinin 1 Monoclonal Antibody (Catalog # MAB8279). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

Detection and quantification of SI protein. (Panels A) and (Panels B) show the concentration-dependence of SI and alpha -actinin signals (Panel C) using automated capillary Western blotting of Caco-2/TC7 lysates. (Panel D) shows Caco-2/TC7 cell surface protein fraction purified after biotinylation from cells cultured on 6-well Transwell filters in glucose or sucrose for 21 days and treated on the apical side for the final 3 days with 1.5 mg/mL OLE. (Panel E) shows the total cellular SI, relative to alpha -actinin with data normalised to the glucose control and presented as mean ± SEM with n = 12, analysed in duplicate runs. The size was determined relative to a protein standard ladder for SI in the total lysate relative to alpha -actinin (F), normalised and presented as mean ± SEM with n = 24. * p < 0.05, ** p < 0.01, *** p < 0.001 from ANOVA followed by Tukey’s post-hoc test. There was no change in the alpha -actinin loading control between the groups. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31266155), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western
• Clone: 910301
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

alpha-Actinin 1 Antibody (910301) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human alpha -Actinin 1
  Asn272-Thr500
  Accession # P12814
• Specificity: Detects human alpha -Actinin 1 in ELISA, and human, mouse and rat alpha -Actinin 1 in Western Blot.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTN1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 2 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha-Actinin 1 Antibody (910301) [Unconjugated]

• actinin 1 smooth muscle
• actinin, alpha 1
• ACTN1
• alpha Actinin 1
• alphaActinin 1
• alpha-Actinin 1
• Alpha-actinin cytoskeletal isoform
• alpha-actinin-1
• BDPLT15
• F-actin cross-linking protein
• FLJ40884
• FLJ54432
• Non-muscle alpha-actinin-1

Background

alpha -Actinin 1 (ACTN1) is a member of the spectrin superfamily with a predicted molecular weight of approximately 100 kDa.  It is 892 amino acids (aa) in length and shares 99% aa identity with mouse and rat ACTN1.  Multiple splice forms exist, resulting in 4 distinct isoforms with different tissue expression patterns.  ACTN1 binds to F-actin as well as other cytoskeletal and signaling proteins and plays an important role in both cell motility and muscle contraction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha-Actinin 1 Antibody (MAB8279)(5)

Publications using MAB8279

• Xiang Li, Mahboubeh Kheirabadi, Patrick G. Dougherty, Kimberli J. Kamer, Xiulong Shen, Nelsa L. Estrella et al. The endosomal escape vehicle platform enhances delivery of oligonucleotides in preclinical models of neuromuscular disorders Molecular Therapy - Nucleic Acids 9/12/2023 [PMID: 37538053]
• Xiang Li, Mahboubeh Kheirabadi, Patrick G. Dougherty, Kimberli J. Kamer, Xiulong Shen, Nelsa L. Estrella et al. The endosomal escape vehicle platform enhances delivery of oligonucleotides in preclinical models of neuromuscular disorders Molecular Therapy - Nucleic Acids 2023-09-12 [PMID: 37538053] (Simple Western, Mouse)
• A Pyner, SY Chan, S Tumova, A Kerimi, G Williamson Indirect Chronic Effects of an Oleuropein-Rich Olive Leaf Extract on Sucrase-Isomaltase In Vitro and In Vivo Nutrients, 2019-07-01;11(7):. 2019-07-01 [PMID: 31266155] (Simple Western, Human)
• Michael J. Houghton, Asimina Kerimi, Vincent Mouly, Sarka Tumova, Gary Williamson Gut microbiome catabolites as novel modulators of muscle cell glucose metabolism The FASEB Journal 2/1/2019 [PMID: 30183376]
• Michael J. Houghton, Asimina Kerimi, Vincent Mouly, Sarka Tumova, Gary Williamson Gut microbiome catabolites as novel modulators of muscle cell glucose metabolism The FASEB Journal 2019-02-01 [PMID: 30183376] (Capillary Immunoblot, Human)

Bioinformatics

• Gene Symbol: ACTN1
• Uniprot:
  • P12814()