alpha 2u-Globulin Antibody [Unconjugated]

a2u-Globulin was detected in perfusion fixed frozen sections of rat liver using 5 µg/mL Goat Anti-Rat a2u-Globulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF586) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

alpha 2u-Globulin Antibody [Unconjugated] Summary

• Immunogen: Rat urine-derived alpha _2u-Globulin
• Specificity: Detects rat alpha _2u-Globulin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha 2u-Globulin Antibody [Unconjugated]

• alpha 2u-Globulin
• AUG
• Mup
• PGCL1

Background

Rat alpha 2u-Globulin (AUG), also known as major urinary protein, belongs to the lipocalin protein family. Under complex hormonal control, alpha 2u-globulin is a low molecular weight protein expressed in a tissue-, age-, and sex-specific fashion. Circulating alpha 2u-globulin is expressed by the liver. It is removed from the plasma by the kidney and excreted.

Rodent studies have employed alpha 2u-globulin nephropathy as an experimental model for the development of kidney tumors. Induction of alpha 2u-globulin nephropathy alters the number, size and shape of hyaline droplets in the proximal tubules. Intra-cellular accumulation of alpha 2u-globulin induces cell death and necrosis of tubular epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha 2u-Globulin Antibody (AF586)(2)

Publications using AF586

• Björn Jacobsen, Christian Freichel, Anne Eichinger-Chapelon, Andreas Brink, Jean-Christophe Hoflack, Mudher Albassam et al. Drug-induced Obstructive and Retrograde Nephropathy Associated with alpha 2u-globulin in Male Rats Toxicologic Pathology 2019-02-01 [PMID: 30587097]
• Yoshiyuki Tago, Min Wei, Naomi Ishii, Anna Kakehashi, Hideki Wanibuchi Evaluation of the Subchronic Toxicity of Dietary Administered Equisetum arvense in F344 Rats Journal of Toxicologic Pathology 2010-12-16 [PMID: 22272034]