alpha 1 Mannosidase 1A Antibody

Western Blot: alpha 1 Mannosidase 1A Antibody [NB600-977] - Whole extract of HepG2 cells was separated on SDS-PAGE and probed with 0.5 ug/mL Rabbit Anti-alpha 1 Mannosidase 1A. The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate.

Immunocytochemistry/Immunofluorescence: alpha 1 Mannosidase 1A Antibody [NB600-977] - NRK cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone (1:1). Fixed cells were stained with 10 ug/mL Rabbit Anti-alpha 1 Mannosidase 1A. The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.

Immunohistochemistry: alpha 1 Mannosidase 1A Antibody [NB600-977] - Immunoperoxidase staining of Anti-alpha 1 Mannosidase 1A in formalin fixed, paraffin-embedded mouse kidney at 0.4 ug/mL.

Western Blot: alpha 1 Mannosidase 1A Antibody [NB600-977] - Whole extract of 3T3 cells was separated on SDS-PAGE and probed with Rabbit Anti-alpha 1 Mannosidase 1A. The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes:1. 1 ug/mL antibody2. 1 ug/mL antibody + alpha 1 Mannosidase 1A immunizing peptide (human, 138-154)

Immunocytochemistry/Immunofluorescence: alpha 1 Mannosidase 1A Antibody [NB600-977] - HepG2 cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone (1:1). Fixed cells were stained with 10 ug/mL Rabbit Anti-alpha 1 Mannosidase 1A. The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.

Immunohistochemistry: alpha 1 Mannosidase 1A Antibody [NB600-977] - Immunoperoxidase staining of Anti-alpha 1 Mannosidase 1A in formalin fixed, paraffin-embedded mouse testis at 0.4 ug/mL.

Immunohistochemistry: alpha 1 Mannosidase 1A Antibody [NB600-977] - Immunoperoxidase staining of Anti-alpha 1 Mannosidase 1A in formalin fixed, paraffin-embedded rat pancreas at 0.2 ug/mL.

Immunohistochemistry: alpha 1 Mannosidase 1A Antibody [NB600-977] - Immunoperoxidase staining of Anti-alpha 1 Mannosidase 1A in formalin fixed, paraffin-embedded rat kidney at 0.2 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.7 mg/ml

alpha 1 Mannosidase 1A Antibody Summary

• Immunogen: Synthetic peptide corresponding to amino acides 138-154 of human alpha 1,2 Mannosidase IA, with N-terminal added cysteine, conjugated to KLH.
• Specificity: Detects alpha 1,2-Mannosidase IA.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAN1A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 5-10 ug/ml
  • Immunohistochemistry
  • Western Blot 0.5-1.0 ug/ml
• Application Notes: Western Blot: Use a whole extract of mouse NIH 3T3 cells, and a chemiluminescence detection agent. Immunofluorescence: Use human HepG2 cells or rat NRK cells.
• Theoretical MW: 73 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4) and 1.0% BSA
• Preservative: 15mM Sodium Azide
• Concentration: 0.7 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for alpha 1 Mannosidase 1A Antibody

• Alpha-1,2-mannosidase IA
• EC 3.2.1
• EC 3.2.1.113
• HUMM3
• HUMM9
• man(9)-alpha-mannosidase
• MAN9
• Man9-mannosidase
• Mannosidase alpha class 1A member 1
• mannosidase, alpha, class 1A, member 1
• mannosyl-oligosaccharide 1,2-alpha-mannosidase IA
• Processing alpha-1,2-mannosidase IA

Background

a1,2-Mannosidase IA is a Type II transmembrane Golgi-resident enzyme that belongs to class I a1,2-Mannosidases (glycosylhydrolase family 47). a1,2-Mannosidases plays an essential role in the maturation of N-glycans to hybrid and complex oligosaccharides in mammalian cells. Class I a1,2- Mannosidases are conserved through evolution. They can be classified into three subgroups according to their enzymatic activities. The first subgroup includes yeast and human endoplasmic reticulum (ER) a1,2-Mannosidases that primarily trim Man9GlcNAc2 to Man8GlcNAc2 isomer B. The second subgroup includes mammalian Golgi a1,2-Mannosidases IA, IB, and IC that trim Man9GlcNAc2 to Man5GlcNAc2 through Man8GlcNAc2 isomer A and C. These Golgi mannosidases display different tissue- and cell-specific expression, subcellular localization, and substrate specificity. The third subgroup includes yeast and mammalian proteins that do not hydrolyze Man9GlcNAc2. Proteins from subgroup 1 and 3 have been implicated in ER quality control and in proteasomal degradation of misfolded glycoproteins. It was also suggested that Golgi mannosidases from the second subgroup may play a role in the ERAD (endoplasmic reticulum-associated degradation) of defective glycoproteins 1-5. Although a1,2-Mannosidase IA is predominantly detected in the juxtanuclear Golgi region by indirect immunofluorescence, significant cell type and speciesdependent variation in localization was reported. The pig liver enzyme has been localized to the ER and transitional vesicles between ER and Golgi, but is not found within the Golgi stacks of porcine hepatocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAN1A1
• Entrez:
  • 4121(Human)
  • 17156(Mouse)
  • 294410(Rat)
• Uniprot:
  • P33908(Human)