SUR-8 Antibody - BSA Free

Western Blot: SUR-8 Antibody [NBP1-87831] - Analysis in human cell line T-47d.

Immunocytochemistry/Immunofluorescence: SUR-8 Antibody [NBP1-87831] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.

Western Blot: SUR-8 Antibody [NBP1-87831] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SUR-8 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LEENKLESLPNEIAYLKDLQKLVLTNNQLTTLPRGIGHLTNLTHLGLGENLLTHLPEEIGTLENLEELYLNDNPNLHSLPFELALCSKLSIMSIENCPLSHLPPQIVA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SHOC2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  SUR-8 Protein (NBP1-87831PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SUR-8 Antibody - BSA Free

• KIAA0862FLJ60412
• Protein soc-2 homolog
• Protein sur-8 homolog
• SIAA0862
• soc-2 (suppressor of clear, C.elegans) homolog
• soc-2 suppressor of clear homolog (C. elegans)
• SOC-2
• SOC2SUR8leucine-rich repeat protein SHOC-2
• SUR-8

Background

Regulatory subunit of protein phosphatase 1 (PP1c) that acts as a M-Ras/MRAS effector and participates in MAPK pathway activation. Upon M-Ras/MRAS activation, targets PP1c to specifically dephosphorylate the 'Ser-259' inhibitory site of RAF1 kinase and stimulate RAF1 activity at specialized signaling complexes

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SUR-8 Antibody (NBP1-87831)(1)

Publication using NBP1-87831

• Yi J, Chen M, Wu X et al. Endothelial SUR-8 acts in an ERK-independent pathway during atrioventricular cushion development. Dev Dyn 2010-07-01 [PMID: 20549726]

Bioinformatics

• Gene Symbol: SHOC2