Alkaline Phosphatase, Intestinal Antibody

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Western Blot: Alkaline Phosphatase, Intestinal Antibody [NB120-7325] - Lane 1: Alkaline Phosphatase (Calf Intestine). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alkaline Phosphatase (Calf Intestine) Antibody at ...read more
Western Blot: Alkaline Phosphatase, Intestinal Antibody [NB120-7325] - Lane 1: Alkaline Phosphatase (Calf Intestine). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alkaline Phosphatase (Calf Intestine) Antibody at ...read more
Western Blot: Alkaline Phosphatase, Intestinal Antibody [NB120-7325] - Lane 1: Calf intestine Alkaline Phosphatase. Load: 1.5 ug Block: 5% Blotto 1 hour at 4C. Primary antibody: diluted to 1:1000 in 5% Blotto overnight ...read more

Product Details

Summary
Reactivity BvSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Catalog# & Conjugate Size Price

Alkaline Phosphatase, Intestinal Antibody Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Alkaline Phosphatase [Calf intestine]
Localization
Attached to the membrane by a GPI-anchor.
Specificity
No cross reactivity is observed against the bacterial enzyme.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ALPI
Purity
Ion exchange chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • ELISA 1:20000-1:100000
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:10-1:500

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Ion exchange chromatography

Alternate Names for Alkaline Phosphatase, Intestinal Antibody

  • alkaline phosphatase, intestinal
  • Alkaline phosphomonoesterase
  • ALPI
  • EC 3.1.3.1
  • Glycerophosphatase
  • IAP
  • Intestinal alkaline phosphatase
  • intestinal-type alkaline phosphatase
  • Kasahara isozyme

Background

Alkaline phosphatase removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes - placental, placental-like, intestinal and non-tissue specific (found in liver, kidney and bone).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ALPI
Entrez