Alkaline Phosphatase/ALPP/ALPI Antibody (rALP/870) - Azide and BSA Free

IHC analysis of formalin-fixed, paraffin-embedded human kidney. Negative tissue control using Alkaline Phosphatase/ALPP/ALPI Antibody (rALP/870) - Azide and BSA Free at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Negative tissue control using Alkaline Phosphatase/ALPP/ALPI Antibody (rALP/870) - Azide and BSA Free at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human heart. Negative tissue control using Alkaline Phosphatase/ALPP/ALPI Antibody (rALP/870) - Azide and BSA Free at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human colon. Negative tissue control using Alkaline Phosphatase/ALPP/ALPI Antibody (rALP/870) - Azide and BSA Free at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human testis. Negative tissue control using Alkaline Phosphatase/ALPP/ALPI Antibody (rALP/870) - Azide and BSA Free at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human placenta stained using Alkaline Phosphatase/ALPP/ALPI Antibody (rALP/870) - Azide and BSA Free at 2ug/ml in PBS for 30min RT.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: rALP/870
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Alkaline Phosphatase/ALPP/ALPI Antibody (rALP/870) - Azide and BSA Free Summary

• Description: Prepared in 10mM PBS WITHOUT BSA & azide at 1.0mg/ml. Also availabe with 0.05% BSA & 0.05% azide.
  
  Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.
• Immunogen: Recombinant full-length human ALPP protein (Uniprot: P05187)
• Localization: Cell membrane
• Specificity: Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALPP
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Alternate Names for Alkaline Phosphatase/ALPP/ALPI Antibody (rALP/870) - Azide and BSA Free

• Alkaline phosphatase Regan isozyme
• alkaline phosphatase, placental type
• Alkaline Phosphatase/ALPP/ALPI
• alkaline phosphomonoesterase
• ALP
• ALPI
• EC 3.1.3.1
• glycerophosphatase
• IAP
• Intestinal alkaline phosphatase
• intestinal-type alkaline phosphatase
• PALP
• Placental alkaline phosphatase 1
• PLAP
• PLAP-1

Background

The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. One of the main sources of this enzyme is the liver, and thus, it's one of several indicators of liver injury in different clinical conditions. In pregnant women, this protein is primarily expressed in placental and endometrial tissue, however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALPP