HAF016). A specific band was detected for Alkaline Phosphatase/ALPP/ALPI at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human placenta tissue and JEG-3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Alkaline Phosphatase/ALPP/ALPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5905) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Alkaline Phosphatase/ALPP/ALPI at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5905-SP | |||
![Western Blot: Alkaline Phosphatase/ALPP/ALPI Antibody (10Q2X7) [NBP3-16373] - Western blot analysis of extracts of PC-3 cells, using Alkaline Phosphatase/ALPP/ALPI Rabbit mAb (NBP3-16373) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-ALPI-Antibody-10Q2X7-Western-Blot-NBP3-16373-img0004.jpg "Western Blot: Alkaline Phosphatase/ALPP/ALPI Antibody (10Q2X7) [NBP3-16373] - Western blot analysis of extracts of PC-3 cells, using Alkaline Phosphatase/ALPP/ALPI Rabbit mAb (NBP3-16373) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: Alkaline Phosphatase/ALPP/ALPI Antibody (10Q2X7) [NBP3-16373] - Western blot analysis of extracts of Mouse testis, using Alkaline Phosphatase/ALPP/ALPI Rabbit mAb (NBP3-16373) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-ALPI-Antibody-10Q2X7-Western-Blot-NBP3-16373-img0005.jpg)
![Alkaline Phosphatase/ALPP/ALPI Antibody (10Q2X7) [NBP3-16373] - Human placenta using Placental alkaline phosphatase (PLAP) antibody at dilution of 1:1000 (40x lens).Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16373_rabbit-alkaline-phosphatase-alpp-alpi-mab-10q2x7-12520239656..jpg)
HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
