ALK/CD246 Antibody (ALK1/6698R) - Azide and BSA Free

Formalin-fixed, paraffin-embedded human lung adenocarcinoma stained with ALK/CD246 Antibody (ALK1/6698R) - Azide and BSA Free at 2ug/ml in PBS. HIER: Tris/EDTA pH9.0; 95°C/45min. 2�CAb: HRP-Poly:30min. DAB:5min.

Formalin-fixed, paraffin-embedded human Anaplastic Large Cell Lymphoma stained with ALK/CD246 Antibody (ALK1/6698R) - Azide and BSA Free at 2ug/ml. HIER: Tris/EDTA pH9.0; 95°C/45min. 2�CAb: HRP-Poly:30min. DAB:5min.

Formalin-fixed, paraffin-embedded human inflammatory myofibroblastic tumor stained with ALK/CD246 Antibody (ALK1/6698R) - Azide and BSA Free at 2ug/ml. HIER: Tris/EDTA pH9.0; 95°C/45min. 2�CAb: HRP-Poly:30min. DAB:5min.

• Reactivity: Hu
• Applications: IHC
• Clone: ALK1/6698R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ALK/CD246 Antibody (ALK1/6698R) - Azide and BSA Free Summary

• Description: Prepared in 10mM PBS WITHOUT BSA & azide at 1.0mg/ml. Also available with 0.05% BSA & 0.05% azide.
  
  Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant human ALK protein fragment around aa 1058-1620 (exact sequence is proprietary) (Uniprot: Q9UM73)
• Localization: Cell membrane
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ALK
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
• Theoretical MW: 177 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Alternate Names for ALK/CD246 Antibody (ALK1/6698R) - Azide and BSA Free

• ALK tyrosine kinase receptor
• ALK
• anaplastic lymphoma kinase (Ki-1)
• Anaplastic lymphoma kinase
• anaplastic lymphoma receptor tyrosine kinase
• CD246 antigen
• CD246
• EC 2.7.10.1
• Ki-1
• NBLST3
• Tcrz

Background

ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. The 2;5 chromosomal translocation occurs in most anaplastic large-cell non-Hodgkin's lymphomas arising from activated T lymphocytes. This rearrangement was shown to fuse the NPM nucleolar phosphoprotein gene on chromosome 5q35 to a previously unidentified protein tyrosine kinase gene, ALK, on chromosome 2p23 (1). In the predicted hybrid protein, the amino terminus of NPM is linked to the catalytic domain of ALK. Immunoblotting with anti-ALK antibody shows that ALK is highly expressed in the neonatal brain (2). Also expressed in the small intestine, testis, and brain but not in normal lymphoid cells, ALK shows greatest sequence similarity to the insulin receptor subfamily of kinases. Unscheduled expression of the truncated ALK may contribute to malignant transformation in these lymphomas (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALK