Aldo-keto Reductase 1C3/AKR1C3 Antibody

Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NB100-1940] - Staining of human breast cells lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NB100-1940] - Staining of paraffin embedded human small intestine. Antibody at 5 ug/mL.Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Aldo-keto Reductase 1C3/AKR1C3 Antibody Summary

• Immunogen: Peptide with sequence CFASHPNYPYSDEY corresponding to C-Terminus according to NP_003730.4.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AKR1C3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.01 - 0.1 ug/mL
• Application Notes: WB: Approx. 35 kDa band observed in human liver and human breast lysates (calculated MW of 36.9 kDa band according to NP_003730).
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Breast Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Aldo-keto Reductase 1C3/AKR1C3 Antibody

• 17-beta-hydroxysteroid dehydrogenase type 5
• 3-alpha-HSD type 2
• 3-alpha-hydroxysteroid dehydrogenase type 2
• AKR1C3
• Aldo-keto Reductase 1C3
• aldo-keto reductase family 1 member C3
• aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II)
• AldoketoReductase 1C3
• Chlordecone reductase homolog HAKRb
• DD3
• DD-3
• DD317-beta-HSD 5
• DDH1
• DDX
• Dihydrodiol dehydrogenase 3
• Dihydrodiol dehydrogenase type I
• dihydrodiol dehydrogenase X
• EC 1.1.1
• EC 1.1.1.112,3-alpha-HSD type II, brain
• EC 1.1.1.188
• EC 1.1.1.213
• EC 1.1.1.63
• EC 1.1.1.64
• EC 1.3.1.20
• HA1753
• HAKRB
• HAKRBtype IIb 3-alpha hydroxysteroid dehydrogenase
• HAKRe
• HSD17B5
• hydroxysteroid (17-beta) dehydrogenase 5
• Indanol dehydrogenase
• KIAA0119hluPGFS
• PGFS
• Prostaglandin F synthase
• Testosterone 17-beta-dehydrogenase 5
• Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase

Background

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aldo-keto Reductase 1C3/AKR1C3 Antibody (NB100-1940)(2)

Publications using NB100-1940

• Werner R, Kulle A, Sommerfeld I et al. Testosterone synthesis in patients with 17beta-hydroxysteroid dehydrogenase 3 deficiency. Sex Dev 2012-01-01 [PMID: 22398356]
• Iczkowski KA, Qiu J, Qian J et al. The dual 5-alpha-reductase inhibitor dutasteride induces atrophic changes and decreases relative cancer volume in human prostate. Urology 2005-01-01 [PMID: 15667867]

Bioinformatics

• Gene Symbol: AKR1C3
• Entrez:
  • 8644(Human)
• Uniprot:
  • P42330(Human)