Human Breast Whole Tissue Lysate (Adult Whole Normal) Summary
Total Protein - Human Adult Normal Tissue: Breast
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression Use in In vitro assay reported in scientific literature (PMID 24480132)
Read 2 Reviews rated 3.5 using NB820-59203 in the following application:
5% non-fat milk in TBS buffer, room temperature for 1 hour
1:3000 in 5% non-fat milk in TBS buffer overnight at 4C, beta-Actin Antibody (8H10D10) NBP1-47423
Rabbit anti-Mouse IgG [HRP]
Secondary Manufacturer Cat#
15-second exposure shows strong band at correct MW.
This human normal breast lysate works well in my western blot, however, it contains a good amount of albumin (blood contamination), which can be seen clearly in the Ponceau S staining. Besides that, I am happy with it.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.