Human Breast Whole Tissue Lysate (Adult Whole Normal)

Images

 
There are currently no images for Breast Lysate (NB820-59203).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, In vitro, IP
Concentration
4.9 mg/ml

Order Details

Human Breast Whole Tissue Lysate (Adult Whole Normal) Summary

Specificity
Total Protein - Human Adult Normal Tissue: Breast

Applications/Dilutions

Application Notes
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression Use in In vitro assay reported in scientific literature (PMID 24480132)
Reviewed Applications
Read 2 Reviews rated 3.5
using
NB820-59203 in the following application:

Publications
Read Publications using NB820-59203.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
g HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
Concentration
4.9 mg/ml

Lysate Details for Array

Type
Tissue
Life Stage
Adult
Tissue
Breast
Protein State
Native
Tissue Condition
Normal
Subcellular Fraction
Whole

Notes

We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for Breast Lysate (NB820-59203)(2)

We have publications tested in 2 applications: IP, in vitro.


Filter By Application
IP
(1)
in vitro
(1)
All Applications
Filter By Species
All Species
Showing Publications 1 - 2 of 2.
Publications using NB820-59203 Applications Species
Swisher LZ, Li J. Development of nanoscale biosensors for cancer related proteasesand blood-borne pathogens based on electrochemicaland optical methods. Thesis. 2015
Swisher LZ, Prior AM, Shishido S et al. Quantitative electrochemical detection of cathepsin B activity in complex tissue lysates using enhanced AC voltammetry at carbon nanofiber nanoelectrode arrays. Biosens Bioelectron 2014 Mar 3 [PMID: 24480132] (IP, in vitro) IP, in vitro

Reviews for Breast Lysate (NB820-59203) (2) 3.52

Average Rating: 3.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB820-59203:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot Breast NB820-59203
Enlarge
reviewed by:
Anonymous
WB Human 10/09/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman Normal Breast Lysate (NB820-59203)
SpeciesHuman
LotA708107

Blocking

Blocking Details5% non-fat milk in TBS buffer, room temperature for 1 hour

Primary Anitbody

Dilution Ratio1:3000 in 5% non-fat milk in TBS buffer overnight at 4C, beta-Actin Antibody (8H10D10) NBP1-47423

Secondary Antibody

Secondary DescriptionRabbit anti-Mouse IgG [HRP]
Secondary Manufacturer Cat#NBP1-73435
Secondary Concentration1:5000

Details

Detection Notes15-second exposure shows strong band at correct MW.

Comments

CommentsThis human normal breast lysate works well in my western blot, however, it contains a good amount of albumin (blood contamination), which can be seen clearly in the Ponceau S staining. Besides that, I am happy with it.
Western Blot Breast NB820-59203
Enlarge
reviewed by:
Anne Frei
WB Human 02/11/2015
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysates
SpeciesHuman

Blocking

Blocking Detailsblocked with 5% milk x 30 min at room temp

Primary Anitbody

Dilution RatioDR1; 1:2000 overnight at 4 deg C in 5% milk

Secondary Antibody

Secondary Descriptiondonkey anti -rabbit IgG HRP
Secondary Manufacturer Cat#GE healthcare NA934V
Secondary Concentration1:5000

Details

Detection NotesECL for 1 min, exposed film for 1 min-20 min. Positive and negative controls worked.

Comments

Commentsran 3 different whole breast lystates and all 3 reacted the same way. Just not positive for our antibody.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Breast Lysate (NB820-59203) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Breast Products

Blogs on Breast

There are no specific blogs for Breast, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

3.5
5
0
4
1
3
1
2
0
1
0

Anonymous
10/09/2015
Application: WB
Species: Human

Anne Frei
02/11/2015
Application: WB
Species: Human