Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB600-914] - Western Blot Results of Rabbit Aldehyde Dehydrogenase 1-A1/ALDH1A1 1-A1/ALDH1A1 antibody. Lane 1: Molecular Weight Marker. Lane 2: Aldehyde Dehydrogenase 1-A1/ALDH1A1.Primary Antibody: Anti-Aldehyde Dehydrogenase 1-A1/ALDH1A1 at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG at 1:40,000 for 30 mins at RT. Block: Blocking buffer for 30 min at RT. Expect MW: ~55kDa.

Western Blot Results of Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody. Lane 1: Molecular Weight Marker. Lane 2: Aldehyde Dehydrogenase. Primary Antibody: Anti-Aldehyde Dehydrogenase at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG

• Reactivity: Ye
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody Summary

• Description: This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Aldehyde Dehydrogenase [Yeast]
  
  Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: Aldehyde Dehydrogenase 1-A1/ALDH1A1 1-A1/ALDH1A1 [Yeast] (Uniprot: P47771)
• Marker: Astrocyte Marker
• Specificity: Cross reactivity against Aldehyde Dehydrogenase 1-A1/ALDH1A1 from other tissues and species may occur but have not been specifically determined.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ALDH1A1
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:20000
  • Immunoprecipitation 1:100
  • Western Blot 1:500-1:2000
• Application Notes: This product has been tested by western blot. Assays should be optimized by the end user for specific conditions for reactivity.

Reactivity Notes

Cross reactivity against Aldehyde Dehydrogenase 1-A1/ALDH1A1 from other tissues and species may occur but have not been specifically determined.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Alternate Names for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

• acetaldehyde dehydrogenase 1
• ALDC
• aldehyde dehydrogenase 1 family, member A1
• aldehyde dehydrogenase 1, soluble
• Aldehyde Dehydrogenase 1A1
• Aldehyde Dehydrogenase 1-A1
• Aldehyde dehydrogenase family 1 member A1
• Aldehyde dehydrogenase, cytosolic
• aldehyde dehydrogenase, liver cytosolic
• ALDH class 1
• ALDH1
• ALDH11
• ALDH1A1
• ALDH-E1
• ALHDII
• EC 1.2.1
• EC 1.2.1.36
• MGC2318
• PUMB1
• RALDH 1
• RALDH1
• retinal dehydrogenase 1
• retinaldehyde dehydrogenase 1

Background

Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALDH1A1
• Uniprot:
  • P47771(Yeast)