Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (M558)

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (M558) [NBP3-23348] - Western blot image of human A431 (lane 1), HepG2 (lane 2), PC3 (lane 3), Jurkat (lane 4), bovine tubulin (lane 5), and recombinant human Aldehyde Dehydrogenase 1-A1/ALDH1A1 (lane 6). The blot was probed with mouse monoclonal Aldehyde Dehydrogenase 1-A1/ALDH1A1 M558 (lanes 1-6) at a dilution of 1:1,000.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: M558
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (M558) Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Clone M558 was generated from a recombinant protein corresponding to amino acids in the N-terminal region from human Aldehyde Dehydrogenase 1-A1/ALDH1A1. This sequence is highly conserved in rat and mouse Aldehyde Dehydrogenase 1-A1/ALDH1A1. [UniProt# P00352]
• Specificity: This antibody antibody detects a 55 kDa protein on SDS-PAGE immunoblots of human A431, HepG2, and mouse liver, as well as human recombinant Aldehyde Dehydrogenase 1-A1/ALDH1A1. The antibody does not detect ALDH1A3 in PC3 cells, ALDH1A2 in Jurkat cells, and bovine tubulin (55 kDa).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALDH1A1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (M558)

• acetaldehyde dehydrogenase 1
• ALDC
• aldehyde dehydrogenase 1 family, member A1
• aldehyde dehydrogenase 1, soluble
• Aldehyde Dehydrogenase 1A1
• Aldehyde Dehydrogenase 1-A1
• Aldehyde dehydrogenase family 1 member A1
• Aldehyde dehydrogenase, cytosolic
• aldehyde dehydrogenase, liver cytosolic
• ALDH class 1
• ALDH1
• ALDH11
• ALDH1A1
• ALDH-E1
• ALHDII
• EC 1.2.1
• EC 1.2.1.36
• MGC2318
• PUMB1
• RALDH 1
• RALDH1
• retinal dehydrogenase 1
• retinaldehyde dehydrogenase 1

Background

Usual human livers contain two major ALDH isozymes, i.e., cytosolic ALDH1 and mitochondrial ALDH2(1). Human red cell aldehyde dehydrogenase (ALDH) resembles the liver cytosolic isozyme in numerous physicochemical properties. Thus, the erythrocyte enzyme, by virtue of its chemical and structural identity with the liver cytosolic enzyme, may serve as a suitable peripheral enzyme model to understand the cause and mechanism of alcohol abuse-related changes in liver cytosolic ALDH that has been found to be reduced in alcoholics (2). ALDH1A1 is known to catalyze the oxidation of retinaldehyde to retinoic acid. Overexpression of human ALDH1A1 in HEK cells led to a more than 20-fold increase in 3-deoxyglucosone dehydrogenase activity (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALDH1A1