ALCAM/CD166 Antibody (2599C) [Unconjugated]

HAF008). A specific band was detected for ALCAM/CD166 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > HAF008). A specific band was detected for ALCAM/CD166 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows lysates of A549 human lung carcinoma cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human ALCAM/CD166 Monoclonal Antibody (Catalog # MAB6563) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for ALCAM/CD166 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." />

Western blot shows lysates of A549 human lung carcinoma cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human ALCAM/CD166 Monoclonal Antibody (Catalog # MAB6563) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (<a class=

F0111). Quadrant markers were set based on control antibody staining (MAB1050). Staining was performed using our Staining Membrane-associated Proteins protocol." > F0111). Quadrant markers were set based on control antibody staining (MAB1050). Staining was performed using our Staining Membrane-associated Proteins protocol." title="HEK293 human embryonic kidney cell line transfected with (A) human ALCAM or (B) irrelevant protein and eGFP was stained with Rabbit Anti-Human ALCAM Monoclonal Antibody (Catalog # MAB6563) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). Quadrant markers were set based on control antibody staining (MAB1050). Staining was performed using our Staining Membrane-associated Proteins protocol." />

HEK293 human embryonic kidney cell line transfected with (A) human ALCAM or (B) irrelevant protein and eGFP was stained with Rabbit Anti-Human ALCAM Monoclonal Antibody (Catalog # MAB6563) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (<a class=

NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surface.Staining was performed using our Fluorescent ICC Staining of Cells on Coverslips Protocol." > NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surface.Staining was performed using our Fluorescent ICC Staining of Cells on Coverslips Protocol." title="ALCAM/CD166 was detected in immersion fixed A431 human epithelial carcinoma cell line using Rabbit Anti-Human ALCAM/CD166 Monoclonal Antibody (Catalog # MAB6563) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surface.Staining was performed using our Fluorescent ICC Staining of Cells on Coverslips Protocol." />

ALCAM/CD166 was detected in immersion fixed A431 human epithelial carcinoma cell line using Rabbit Anti-Human ALCAM/CD166 Monoclonal Antibody (Catalog # MAB6563) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; <a class=

• Applications: WB, Flow, CyTOF-ready, ICC/IF
• Clone: 2599C
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

ALCAM/CD166 Antibody (2599C) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ALCAM/CD166
  Trp28-Ala526
  Accession # Q13740
• Specificity: Detects human ALCAM/CD166 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 3-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ALCAM/CD166 Antibody (2599C) [Unconjugated]

• activated leucocyte cell adhesion molecule
• activated leukocyte cell adhesion moleculeFLJ38514
• ALCAM
• CD166 antigen
• CD166
• MEMDMGC71733

Background

ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. The ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains.

1. Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
2. Aruffo, A. et al. (1997) Immunol. Today 18:498.
3. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q13740()