ALCAM/CD166 Antibody (2599C) [Unconjugated]

Western blot shows lysates of A549 human lung carcinoma cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human ALCAM/CD166 Monoclonal Antibody (Catalog # MAB6563) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for ALCAM/CD166 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HEK293 human embryonic kidney cell line transfected with (A) human ALCAM or (B) irrelevant protein and eGFP was stained with Rabbit Anti-Human ALCAM Monoclonal Antibody (Catalog # MAB6563) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). Quadrant markers were set based on control antibody staining (MAB1050). Staining was performed using our Staining Membrane-associated Proteins protocol.

ALCAM/CD166 was detected in immersion fixed A431 human epithelial carcinoma cell line using Rabbit Anti-Human ALCAM/CD166 Monoclonal Antibody (Catalog # MAB6563) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surface.Staining was performed using our Fluorescent ICC Staining of Cells on Coverslips Protocol.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready, ICC/IF
• Clone: 2599C
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

ALCAM/CD166 Antibody (2599C) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ALCAM/CD166
  Trp28-Ala526
  Accession # Q13740
• Specificity: Detects human ALCAM/CD166 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 3-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ALCAM/CD166 Antibody (2599C) [Unconjugated]

• activated leucocyte cell adhesion molecule
• activated leukocyte cell adhesion moleculeFLJ38514
• ALCAM
• CD166 antigen
• CD166
• MEMDMGC71733

Background

ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. The ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains.

1. Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
2. Aruffo, A. et al. (1997) Immunol. Today 18:498.
3. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q13740()