AKT1 RNAi

Images

 
Western Blot: Akt1 RNAi [H00000207-R02V] - Analysis of AKT1 over-expressed 293 cell line. Lane 2 cotransfected with AKT1 Validated Chimera RNAi Lane 1 non-transfected control. Blot probed with H00000207-M01. GAPDH ( ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, RNAi, RNAi SP

Order Details

AKT1 RNAi Summary

Specificity
Homo sapiens v-akt murine thymoma viral oncogene homolog 1, mRNA
Gene
AKT1

Applications/Dilutions

Application Notes
This RNAi causes effective protein knockdown which has been antibody validated.
Reviewed Applications
Read 1 Review rated 5
using
H00000207-R02V in the following application:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for AKT1 RNAi

  • AKT
  • Akt1
  • EC 2.7.11
  • EC 2.7.11.1
  • PKB alpha
  • PKBMGC99656
  • PRKBA
  • Protein kinase B
  • Proto-oncogene c-Akt
  • rac protein kinase alpha
  • RAC-alpha serine/threonine-protein kinase
  • RAC-alpha
  • RAC-PK-alpha
  • RACPKB-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1

Background

Position of the Chimera RNAi. The related RNAi products listed were designed from different accesion number but sharing the same RNAi sequence. Chimera RNA interference (chimera RNAi) is process by which small interfering RNA/DNA chimera triggers the destruction of mRNA for the original gene. The discovery work, design, and application of chimera RNAi has been pioneered by Professor Kaoru Saigo and Dr. Kumiko Ui-Tei at the University of Tokyo. Chimera RNAi has many advantages over the conventional siRNAs. First, it has been demonstrated to have reliable knock-down for over 10,000 human genes. Because the human genome is composed of an intricate, genetic network, chimera RNAi's unique design has successfully obviated the off-target effects including microRNA-based influence. Another advantage of the chimera RNAi technology is its effectiveness at low concentrations (0.5nM to 5nM); only mRNA is destroyed so genomic genes are not affected. Finally, having both the sense and anti-sense strands consisting RNA/DNA chimera, it offers much greater compound stability for streamlining in vitro and in vivo assays and applications while minimizing interferon induction and other adverse reactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. RNAi are guaranteed for 3 months from date of receipt.

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Publications for AKT1 RNAi (H00000207-R02V) (0)

There are no publications for AKT1 RNAi (H00000207-R02V).
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Review for AKT1 RNAi (H00000207-R02V) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using H00000207-R02V:
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Images Ratings Applications Species Date Details
Western Blot AKT1 H00000207-R02V
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reviewed by:
Ronald Miller
WB Human 12/12/2014
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Summary

ApplicationWestern Blot
Sample TestedCell lysate from HeLa cells
SpeciesHuman

Blocking

Blocking Details5% BSA in 1X PBS

Primary Anitbody

Dilution Ratio1:1000 in 1X PBS with troton X-100 (0.1%) at 4 degree O/N

Secondary Antibody

Secondary DescriptionGoat Anti-mouse IgG - HRP (Life Tech)
Secondary Manufacturer Cat#A24524
Secondary Concentration1:5K

Details

Detection NotesECL, 1 min exposure with film

Comments

CommentsI could get approx 90-95% silencing of AKT proteins.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for AKT1 RNAi (H00000207-R02V). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for a antibody that recognizes human Akt1 but NOT Akt2 or 3, for Western blot analyses. I also want that antibody to recognize Akt1 regardless of its phosphorylated form.
    • At the moment we do not have an AKT1 antibody that definitively does not react with either AKT2 or AKT3.

Additional AKT1 Products

Bioinformatics Tool for AKT1 RNAi (H00000207-R02V)

Discover related pathways, diseases and genes to AKT1 RNAi (H00000207-R02V). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for AKT1 RNAi (H00000207-R02V)

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Pathways for AKT1 RNAi (H00000207-R02V)

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PTMs for AKT1 RNAi (H00000207-R02V)

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Research Areas for AKT1 RNAi (H00000207-R02V)

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Blogs on AKT1. Showing 1-10 of 11 blog posts - Show all blog posts.

Using a STAT3 antibody in chromatin immunoprecipitation (ChIP)
Signal transducer and activator of transcription 3 (STAT3) is an important oncogenic transcriptional factor that mediates tumor induced immune suppression.  Specifically, STAT3 transmits signals from cytokines and growth factor receptors in the pla...  Read full blog post.

Altered expression of BCL2 in cancer
Similar to other cell processes, the balance between cell survival and cell death is an important equilibrium that when altered expression of genes can lead to a variety of disease.  For example, too little cell death can promote cell overgrowth a...  Read full blog post.

AKT1 - Regulating cell growth and survival through phosphorylation
AKT1 is a serine/threonine protein kinase with homology to protein kinase A (PKA) and protein kinase C (PKC). AKT1 contains the central kinase domain sandwiched between a pleckstrin homology domain and a regulatory domain (1). AKT1 is regulated by...  Read full blog post.

Akt1 - a central player in cell survival signaling
Akt1 is one of three isoforms of Akt belonging to the AGC family of serine/threonine kinases (Akt1, Akt2, and Akt3). All Akt isoforms contain an N-terminal Plekstrin Homology (PH) domain, a C-terminal regulatory domain, and a central catalytic kin...  Read full blog post.

AKT1, Scene 1: The Cell Must Go On
Akt1 is a serine/threonine-specific protein kinase involved in many cellular signaling pathways. The major function of this kinase is to mediate cell survival, but it also plays key roles in various other cellular functions such as glycogen synthesis...  Read full blog post.

Caspase 9 and Mitochondrial Apoptosis Regulation
Caspase 9 (also termed ICE-LAP6, Mch6, Apaf-3) is a member of cysteine protease family of caspases and is encoded by the CASP9 gene in humans. Caspase-9 is involved in mitochondrial apoptosis pathway and is an initiator caspase. Pro-caspase-9 is activ...  Read full blog post.

Mapping Signal Transduction with mTOR Antibodies
The protein encoded by mTOR (mammalian target of rapamycin), also known as dTOR in Drosophila, belongs to a family of phosphatidylinositol kinase-related kinases. These kinases regulate fundamental processes of cell growth, proliferation, metabolism...  Read full blog post.

EZH2: Epigenetic Regulation Made Easy!
Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i...  Read full blog post.

Antibodies Targeting the mTOR Pathway for Many Diseases
The mammalian target of rapamycin (mTOR) is a downstream effector of the phosphatidylinositol 3-kinase (PI3K)/Akt (protein kinase B) signaling pathway that mediates cell survival and proliferation. It is increasingly apparent that mTOR signaling impac...  Read full blog post.

AKT Antibody Assays: A Complex Area with an Easy Solution
We at Novus Biologicals place a lot of emphasis on the kinase signaling pathways. Kinases, or phosphotransferase enzymes play a key role in phosphorylation signaling. Over 500 human protein kinases have so far been discovered. They play essential role...  Read full blog post.

Showing 1-10 of 11 blog posts - Show all blog posts.

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Recent Reviews

5
5
1
4
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0
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1
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Ronald Miller
12/12/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol AKT1
Entrez