Recombinant Mouse AKR1B1 His Protein

SDS-Page: AKR1B1 Recombinant Protein [NBP2-51989] - 15% SDS Page (3 ug)

• Reactivity: Mu
• Applications: PAGE, Enzyme Activity
• Concentration: 1 mg/ml

Recombinant Mouse AKR1B1 His Protein Summary

• Description: A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-316 of Mouse AKR1B1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMASHLEL NNGTKMPTLG LGTWKSPPGQ VTEAVKVAID LGYRHIDCAQ VYQNEKEVGV ALQEKLKEQV VKRQDLFIVS KLWCTFHDKS MVKGAFQKTL SDLQLDYLDL YLIHWPTGFK PGPDYFPLDA SGNVIPSDTD FVDTWTAMEQ LVDEGLVKTI GVSNFNPLQI ERILNKPGLK YKPAVNQIEC HPYLTQEKLI EYCHSKGIVV TAYSPLGSPD RPWAKPEDPS LLEDPRIKAI AAKYNKTTAQ VLIRFPIQRN LVVIPKSVTP VRIAENLKVF DFEVSSEDMA TLLSYNRNWR VCALMSCAKH KDYPFHAEV

• Details of Functionality: Specific activity is > 500 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction of 1.0 pmole DL-glyceraldehyde in the presence of NADPH per minute at pH7.0 at 37C.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: AKR1B1
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 38.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Mouse AKR1B1 His Protein

• ADR
• Aldehyde reductase
• Aldo-keto reductase family 1 member B1
• aldo-keto reductase family 1, member B1 (aldose reductase)
• ALDR1aldose reductase
• ALR2
• ARaldehyde reductase 1
• EC 1.1.1
• EC 1.1.1.21
• Lii5-2 CTCL tumor antigen
• low Km aldose reductase
• MGC1804

Background

Aldose reductase (also designated AKR1B1, ALDR1, ALR2 or AR) is member of the monomeric NADPH-dependent aldoketoreductase family. Aldose reductase, which has a molecular mass of 36 kDa, catalyzes the reduction of various aldehydes and has been implicated in the development of diabetic complications by catalyzing the reduction of the aldehyde form of glucose, to the corresponding sugar alcohol, sorbitol. This pathway plays a minor role in glucose metabolism in most tissues, however in diabetic hyperglycemia, cells undergoing insulin-independent uptake of glucose accumulate significant quantities of sorbitol. The resulting hyperosmotic stress to cells may be a cause of diabetic complications such as neuropathy, retinopathy, and cataracts. Aldose reductase is very similar to human aldehyde reductase, bovine prostaglandin F synthase and to the European common frog protein, rho-crystallin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: AKR1B1