AK3L1 Antibody

Western Blot: AK3L1 Antibody [NBP2-15317] - Sample (30 ug of whole cell lysate) A: HeLa B: HepG2 C: HCT116 12% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: AK3L1 Antibody [NBP2-15317] - AK3L1 antibody detects Adenylate kinase 4 protein at mitochondria by immunofluorescent analysis. Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37C for 30 min. Green: AK3L1 protein stained by AK3L1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: AK3L1 Antibody [NBP2-15317] - AK3L1 antibody detects AK3L1 protein at mitochondria in mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. AK3L1 antibody diluted at 1:500.

Immunohistochemistry-Paraffin: AK3L1 Antibody [NBP2-15317] - Paraffin-embedded Colon ca, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: AK3L1 Antibody [NBP2-15317] - Paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

AK3L1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human AK3L1. The exact sequence is proprietary.
• Localization: Mitochondrion matrix
• Predicted Species: Bovine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AK4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for AK3L1 Antibody

• adenylate kinase 3
• adenylate kinase 3-like 1
• Adenylate kinase 3-like
• adenylate kinase 4
• AK 4
• AK3
• AK3adenylate kinase isoenzyme 4, mitochondrial
• AK3L1
• AK3L2
• AK4
• ATP-AMP transphosphorylase
• EC 2.7.4
• EC 2.7.4.3
• GTP:AMP phosphotransferase
• MGC166959
• mitochondrial adenylate kinase-3
• nucleoside-triphosphate-adenylate kinase

Background

This gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: AK4
• Uniprot:
  • P27144(Human)