AK2 Antibody

Images

 
Western Blot: AK2 Antibody [NBP1-33160] - Sample (30 ug of whole cell lysate) A: H1299 B: Hep G2 12% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: AK2 Antibody [NBP1-33160] - Analysis of methanol-fixed HeLa, using Adenylate kinase 2 antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: AK2 Antibody [NBP1-33160] - Paraffin-embedded rat liver. Adenylate kinase 2 antibody [N1C2] diluted at 1:500.
Immunohistochemistry-Paraffin: AK2 Antibody [NBP1-33160] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Rt, Mu, Bv, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

AK2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Adenylate kinase 2. The exact sequence is proprietary.
Localization
Mitochondrion intermembrane space
Predicted Species
Mouse (96%), Rhesus Macaque (100%), Bovine (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AK2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (83%), Xenopus laevis (82%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AK2 Antibody

  • adenylate kinase 2
  • adenylate kinase 2, mitochondrial
  • adenylate kinase isoenzyme 2, mitochondrial
  • ADK2
  • AK 2
  • ATP-AMP transphosphorylase 2
  • EC 2.7.4
  • EC 2.7.4.3

Background

Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Two transcript variants encoding distinct isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional AK2 Products

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Bioinformatics

Gene Symbol AK2