AK2 Antibody - BSA Free

Western Blot: AK2 Antibody [NBP1-33160] - Wild-type (WT) and AK2 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with AK2 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: AK2 Antibody [NBP1-33160] - Analysis of methanol-fixed HeLa, using Adenylate kinase 2 antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: AK2 Antibody [NBP1-33160] - Paraffin-embedded rat liver. Adenylate kinase 2 antibody [N1C2] diluted at 1:500.

Western Blot: AK2 Antibody [NBP1-33160] - Sample (30 ug of whole cell lysate) A: H1299 B: Hep G2 12% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: AK2 Antibody [NBP1-33160] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.

• Reactivity: Hu, Rt, Mu, Bv, RM
• Applications: WB, ICC/IF, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

AK2 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human AK2. The exact sequence is proprietary.
• Localization: Mitochondrion intermembrane space
• Predicted Species: Mouse (96%), Rhesus Macaque (100%), Bovine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AK2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (83%), Xenopus laevis (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for AK2 Antibody - BSA Free

• adenylate kinase 2
• adenylate kinase 2, mitochondrial
• adenylate kinase isoenzyme 2, mitochondrial
• ADK2
• AK 2
• AK2
• ATP-AMP transphosphorylase 2
• EC 2.7.4
• EC 2.7.4.3

Background

Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Two transcript variants encoding distinct isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: AK2