Aminoacylase/ACY1 Antibody (4F1-B7)

Western Blot: Aminoacylase/ACY1 Antibody (4F1-B7) [H00000095-M01] - Analysis of ACY1 expression in transfected 293T cell line by ACY1 monoclonal antibody (M01), clone 4F1-B7.Lane 1: ACY1 transfected lysate(45.9 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: Aminoacylase/ACY1 Antibody (4F1-B7) [H00000095-M01] - Analysis of monoclonal antibody to ACY1 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: Aminoacylase/ACY1 Antibody (4F1-B7) [H00000095-M01] - Analysis of monoclonal antibody to ACY1 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. Antibody concentration 3 ug/ml.

Western Blot: Aminoacylase/ACY1 Antibody (4F1-B7) [H00000095-M01] - ACY1 monoclonal antibody (M01), clone 4F1-B7. Analysis of ACY1 expression in K-562.

Immunoprecipitation: Aminoacylase/ACY1 Antibody (4F1-B7) [H00000095-M01] - Analysis of ACY1 transfected lysate using anti-ACY1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ACY1 MaxPab rabbit polyclonal antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clone: 4F1-B7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Aminoacylase/ACY1 Antibody (4F1-B7) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ACY1 (AAH00545, 1 a.a. ~ 408 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTSKGPAEEHPSVTLFRQYLRIRTVQPKPDYGAAVAFFEETARQLGLGCQKVEVAPGYVVTVLTWPGTNPTLSSILLNSHTDVVPVFKEHWSHDPFEAFKDSEGYIYARGAQDVKCVSIQYLEAVRRLKVEGHRFPRTIHMTFVPDEEVGGHQGMELFVQRPEFHALRAGFALDEGIANPTDAFTVFYSERSPWWVRVTSTGRPGHASRFMEDTAAEKLHKVVNSILAFREKEWQRLQSNPHLKEGSVTSVNLTKLEGGVAYNVIPATMSASFDFRVAPDVDFKAFEEQLQSWCQAAGEGVTLEFAQKWMHPQVTPTDDSNPWWAAFSRVCKDMNLTLEPEIMPAATDNRYIRAVGVPALGFSPMNRTPVLLHDHDERLHEAVFLRGVDIYTRLLPALASVPALPSDS
• Specificity: ACY1 - aminoacylase 1
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACY1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recominant protein and cell lysate for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Aminoacylase/ACY1 Antibody (4F1-B7)

• ACY1
• ACY-1
• ACY1D
• acylase
• aminoacylase 1
• Aminoacylase
• aminoacylase-1
• EC 3.5.1
• EC 3.5.1.14
• N-acyl-L-amino-acid amidohydrolase

Background

Aminoacylase-1 is a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. ACY1 has been assigned to chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and ACY1 is the first member of a new family of zinc-binding enzymes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aminoacylase/ACY1 Antibody (H00000095-M01)(3)

Publications using H00000095-M01

• Ataman-onal Y, Beaulieu C, Busseret S et al. Prodefensin-A6 Assay Method for The In Vitro Diagnosis of Colorectal Cancer. United States Patent Application 2015-09-10
• Alessandri MG, Casarano M, Pezzini I et al. Isolated Mild Intellectual Disability Expands the Aminoacylase 1 Phenotype Spectrum. JIMD Rep 2014-07-06 [PMID: 24997716] (WB, ICC/IF, Human)
  
  Details:
  Aminoacylase 1/ ACY1 antibody used for WB (dilution 1:100) and ICC-IF on cultured skin fibroblasts from human child Mild Intellectual Disability patient and a normal control.
• Jansen FH, Rijswijk A, Teubel W et al. Profiling of antibody production against xenograft-released proteins by protein microarrays discovers prostate cancer markers. J Proteome Res. 2011-12-02 [PMID: 22136385]

Bioinformatics

• Gene Symbol: ACY1
• Entrez:
  • 95(Human)
• OMIM:
  • 104620(Human)
• Uniprot:
  • AAH00545(Human)