AIF Antibody

Product Discontinued

AIF Antibody Summary

• Immunogen: This antibody was generated by immunizing rabbits with synthetic peptides of human AIF (NP_001124319).
• Specificity: A 65 kDa band is observed.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AIFM1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 23824039)
• Control:

  Mouse Heart Whole Tissue Lysate (Adult Whole Normal)
  K-562 Whole Cell Lysate
  AIF-1/Iba1 Overexpression Lysate
  Human Heart Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Human, Mouse, Rat Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for AIF Antibody

• AIF
• AIFapoptosis-inducing factor 1, mitochondrial
• AIFM1
• apoptosis-inducing factor, mitochondrion-associated, 1
• COXPD6
• MGC111425
• PDCD8
• PDCD8striatal apoptosis-inducing factor
• programmed cell death 8 (apoptosis-inducing factor)
• Programmed cell death protein 8

Background

Apoptosis-inducing factor (AIF) is a phylogenetically ancient mitochondrial intermembrane flavoprotein. AIF induces caspase-independent peripheral chromatin condensation and large-scale DNA fragmentation when added to purified nuclei. Upon apoptosis induction AIF translocates to the cytosol and the nucleus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AIF Antibody (NB100-55774)(2)

Publications using NB100-55774

• Sahu Upasana, Sidhar Himakshi, Ghate Pankaj S et al. A Novel Anticancer Agent, 8-Methoxypyrimido[4',5':4,5]thieno(2,3-) Quinoline-4(3H)-One Induces Neuro 2a Neuroblastoma Cell Death through p53-Dependent, Caspase-Dependent and -Independent Apoptotic Pathways. PLoS One. 2013-01-01 [PMID: 23824039] (ICC/IF)
• Mohan J, Gandhi AA, Bhavya BC et al. Caspase-2 triggers Bax-Bak-dependent and -independent cell death in colon cancer cells treated with resveratrol. J Biol Chem. 2006-06-30 [PMID: 16617056]
  
  Details:
  Caspase-3 (IMG-144) [WB, Fig 1C, Fig 7C, Fig 8 (human colon adenocarcinoma cell line, HCT 116)] 2. AIF (IMG-303) [WB, Fig 2B, IF/FCC, Fig 2B (HCT 116 cells)] 3. RNAi vector (IMG-805) [ Fig 3CD (HCT 116 cells)].

Bioinformatics

• Gene Symbol: AIFM1
• Entrez:
  • 9131(Human)
  • 26926(Mouse)
  • 29427(Rat)
• Uniprot:
  • O95831(Human)