Mouse Heart Whole Tissue Lysate (Adult Whole Normal)

Western Blot: Mouse Heart Whole Tissue Lysate (Adult Normal) [NB820-59660] - Western blot analysis of GAPDH (NB300-323) using whole normal mouse heart lysate [NB820-59660].

SDS-Page: Mouse Heart Whole Tissue Lysate (Adult Normal) [NB820-59660] - Ponceau S staining of whole normal mouse heart lysate using NB820-59660.

• Reactivity: Mu
• Applications: WB, ELISA
• Concentration: 5.0 mg/ml

Mouse Heart Whole Tissue Lysate (Adult Whole Normal) Summary

• Description: We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.
• Specificity: Heart

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression. Use in ELISA reported in scientific literature (PMID:35860280).

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
• Concentration: 5.0 mg/ml

Lysate Details for Array

• Type: Tissue
• Life Stage: Adult
• Tissue: Heart
• Protein State: Native
• Tissue Condition: Normal
• Subcellular Fraction: Whole

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for Heart Lysate (NB820-59660)(1)

Publication using NB820-59660

• Elshikha AS, Teng XY, Kanda N et al. TLR7 Activation Accelerates Cardiovascular Pathology in a Mouse Model of Lupus Frontiers in immunology 2022-07-04 [PMID: 35860280] (ELISA)