![ELISA: Rat AHR ELISA Kit (Chemiluminescence) [NBP2-66671] - Samples were spiked with high concentrations of Rat AHR and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-66671_rat-ahr-elisa-kit-chemiluminescence-13220241612130.jpg "ELISA: Rat AHR ELISA Kit (Chemiluminescence) [NBP2-66671] - Samples were spiked with high concentrations of Rat AHR and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
| Reactivity | RtSpecies Glossary |
| Applications | ELISA |
| Suitable Sample Type | Serum, plasma and other biological fluids |
| Standard Curve Range | 62.50-4000 pg/mL |
| Sensitivity | 37.50 pg/mL |
| Specificity | This kit recognizes Rat AhR in samples. No significant cross-reactivity or interference between Rat AhR and analogues was observed. |
| Standard Curve Range | 62.50-4000 pg/mL |
| Sensitivity | 37.50 pg/mL |
| Assay Type | Sandwich-CLIA |
| Inter-Assay | CV% < 9.62% |
| Intra-Assay | CV% < 10.55% |
| Spike Recovery | 86-113% |
| Sample Volume | 100 uL |
| Kit Type | ELISA Kit (Chemiluminescence) |
| Gene | AHR |
| Storage | Storage of components varies. See protocol for specific instructions. |
| Components |
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Research Areas for AHR ELISA Kit (NBP2-66671)Find related products by research area.
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AHR - A transcription factor regulating immunity and tumorigenesis The aryl hydrocarbon receptor (AHR) is a ligand activated transcription factor that controls the expression of a diverse set of genes. In the absence of ligand, AHR is retained in the cytoplasm. Upon ligand binding AHR translocates to the nucleus w... Read full blog post. |
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Aryl Hydrocarbon Signaling: AIP, AhR, ARNT, BMAL1 and more... AH receptor-interacting protein (AIP) is a 37 kD immunophilin-like factor found in a variety of tissues with expression levels ranging from high (spleen, thymus, pituitary heart, placenta and skeletal muscle) to low (liver, kidney and lung). It mediat... Read full blog post. |
| Gene Symbol | AHR |
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![Immunocytochemistry/Immunofluorescence: AHR Antibody [NB100-128] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti AHR [NB100-128] at a 1:100 dilution overnight at 4C and detected with an anti-goat DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](https://images.novusbio.com/images/AHR-Antibody-Immunocytochemistry-Immunofluorescence-NB100-128-img0004.jpg "Immunocytochemistry/Immunofluorescence: AHR Antibody [NB100-128] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti AHR [NB100-128] at a 1:100 dilution overnight at 4C and detected with an anti-goat DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")