AHR Antibody (3B12) - Azide and BSA Free

Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR expression in transfected 293T cell line by AHR monoclonal antibody (M02), clone 3B12.Lane 1: AHR transfected lysate(96.147 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: AHR Antibody (3B12) [H00000196-M02] - Analysis of monoclonal antibody to AHR on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR expression in COLO 320 HSR ( Cat # L020V1 ).

Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR over-expressed 293 cell line, cotransfected with AHR Validated Chimera RNAi ( Cat # H00000196-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with AHR monoclonal antibody (M02), clone 3B12 (Cat # H00000196-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Immunoprecipitation: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR transfected lysate using anti-AHR monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with AHR MaxPab rabbit polyclonal antibody.

ELISA: AHR Antibody (3B12) [H00000196-M02] - Detection limit for recombinant GST tagged AHR is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IP, ChIP, KD
• Clone: 3B12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

AHR Antibody (3B12) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: AHR (NP_001612, 721 a.a. ~ 820 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGSFEPSPYPTTSSLEDFVTCLQLPENQKHGLNPQSAIITPQTCYAGAVSMYQCQPEPQHTHVGQMQYNPVLPGQQAFLNKFQNGVLNETYPAELNNINN
• Specificity: aryl hydrocarbon receptor
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: AHR
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA 0.03ng/ml
  • Immunocytochemistry/ Immunofluorescence 20ug/mL
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for IF, RNAi Validation, and ELISA. ChIP usage reported in scientific literature (PMID: 23714156).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for AHR Antibody (3B12) - Azide and BSA Free

• Ah receptor
• AHR
• AH-receptor
• aryl hydrocarbon receptor
• BHLHE76
• bHLHe76aromatic hydrocarbon receptor
• Class E basic helix-loop-helix protein 76

Background

This gene encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Its ligands included a variety of aromatic hydrocarbons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AHR Antibody (H00000196-M02)(5)

Publications using H00000196-M02

• Kalthoff S, Landerer S, Reich J, Strassburg CP et al. Protective effects of coffee against oxidative stress induced by the tobacco carcinogen benzo[a]pyrene. Free Radic Biol Med 2017-03-12 [PMID: 28300668]
• Vogel CF, Khan EM, Leung PS et al. Cross-talk between Aryl Hydrocarbon Receptor and the inflammatory response: a Role for NF-kB. J Biol Chem. 2013-12-03 [PMID: 24302727]
• Kalthoff S, Winkler A, Freiberg N et al. Gender matters: estrogen receptor alpha (ERalpha) and histone deacetylase (HDAC) 1 and 2 control the gender-specific transcriptional regulation of human uridine diphosphate glucuronosyltransferases genes (UGT1A). J Hepatol. 2013-10-01 [PMID: 23714156] (Chemotaxis, IP, Human)
• Vlachos C, Schulte BM, Magiatis P et al. Malassezia-derived indoles activate the aryl hydrocarbon receptor and inhibit Toll-like receptor-induced maturation in monocyte-derived dendritic cells. Br J Dermatol. 2012-04-19 [PMID: 22533375]
• Schults MA, Timmermans L, Godschalk RW et al. Diminished carcinogen detoxification is a novel mechanism for hypoxia-inducible factor 1-mediated genetic instability. J Biol Chem;285(19):14558-64. 2010-05-07 [PMID: 20228066]

Bioinformatics

• Gene Symbol: AHR
• Entrez:
  • 196(Human)
• OMIM:
  • 600253(Human)
• Uniprot:
  • NP_001612(Human)