Aggrecan Neoepitope Antibody [DyLight 650]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 650

Order Details

Aggrecan Neoepitope Antibody [DyLight 650] Summary

Immunogen
A synthetic peptide corresponding to a region of human Aggrecan (within residues 350-400). [UniProt# P16112]
Specificity
Aggrecan Neo (JSCNIT)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACAN
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
This Aggrecan Neoepitope antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-paraffin embedded sections, and Western Blot. In WB a band is detected at ~61 kDa representing the neoepitope sequence (NITEGE) generated by aggrecanase-mediated cleavage at Glu373-Ala374 of aggrecan core proteins. Use in Immunohistochemistry-Frozen and Flow Cytometry reported in scientific literature (PMID 21482542)

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Aggrecan Neoepitope Antibody [DyLight 650]

  • AGC1SEDK
  • aggrecan core protein
  • aggrecan
  • Cartilage-specific proteoglycan core protein
  • Chondroitin sulfate proteoglycan 1
  • Chondroitin sulfate proteoglycan core protein 1
  • CSPCP
  • CSPG1aggrecan 1
  • CSPGCP
  • large aggregating proteoglycan
  • MSK16AGCAN

Background

Aggrecan is a member of large, aggregating proteoglycans (also including, versican, brevican and neurocan) found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMP?s) and aggrecanase (ADAMTS). Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. The C-terminal NITEGE fragment is one of the G1 fragments produced when aggrecan is cleaved with aggrecanase. It is the most abundant fragment found in normal human synovial fluid and articular cartilage, and is the major product produced by aggrecanase cleavage. The NITEGE fragment has also been shown to accumulate rapidly after an acute cartilage injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Aggrecan Neoepitope Antibody (NB100-74350C) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-74350AF350
Alexa Fluor 405 NB100-74350AF405
Alexa Fluor 488 NB100-74350AF488
Alexa Fluor 532 NB100-74350AF532
Alexa Fluor 594 NB100-74350AF594
Alexa Fluor 647 NB100-74350AF647
Alexa Fluor 700 NB100-74350AF700
Alexa Fluor 750 NB100-74350AF750
Allophycocyanin/Cy7 NB100-74350APCCY7
Biotin NB100-74350B
DyLight 350 NB100-74350UV
DyLight 405 NB100-74350V
DyLight 488 NB100-74350G
DyLight 550 NB100-74350R
DyLight 594 NB100-74350DL594
DyLight 650 NB100-74350C
DyLight 680 NB100-74350FR
DyLight 755 NB100-74350IR
FITC NB100-74350F
HRP NB100-74350H
Janelia Fluor 549 NB100-74350JF549
Janelia Fluor 646 NB100-74350JF646
PE/Atto594 NB100-74350PEATT594
PE/Cy5.5 NB100-74350PECY55
PE/Cy7 NB100-74350PECY7

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Bioinformatics

Gene Symbol ACAN