AF10 Antibody

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Western Blot: AF10 Antibody [NBP1-49867] - Western blot analysis of AF10 in Rat Testis lysate (35ug protein in RIPA buffer) using NBP1-49867 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

AF10 Antibody Summary

Immunogen
Peptide with sequence C-RLEDTTARFTNAN corresponding to internal region according to NP_001009569.1.
Epitope
RLEDTTARFTNAN - internal region
Specificity
This antibody is expected to recognize both reported isoforms (NP_004632.1; NP_001009569.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MLLT10
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Experiments gave bands at approx. 140 kDa band and 60 kDa band in rat testis lysates. These bands correspond to earlier findings with different antibodies from other sources. This protein has a calculated MW of 109 kDa band according to human NP_004632.1, 107 kDa band according to human NP_001009569.1and 103 kDa band according to rat NP_001012162.1.
Publications
Read Publication using NBP1-49867.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human, Mouse, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AF10 Antibody

  • AF10MGC75086
  • ALL1-fused gene from chromosome 10 protein
  • DKFZp686E10210
  • myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 10
  • myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 10
  • protein AF-10
  • type I AF10 protein

Background

AF10 is a putative transcription factor containing a leucine zipper motif. A t(10;11)(p12;q23) chromosomal translocation present in a proportion of acute myeloid leukaemias results in expression of an AF10 (carboxy terminus) MLL (amino terminus) fusion protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AF10 Antibody (NBP1-49867)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MLLT10
Entrez
Uniprot