AF10 Antibody

Western Blot: AF10 Antibody [NBP1-49867] - Western blot analysis of AF10 in Rat Testis lysate (35ug protein in RIPA buffer) using NBP1-49867 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence

• Reactivity: Rt, Hu, Mu, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

AF10 Antibody Summary

• Description: Novus Biologicals Goat AF10 Antibody (NBP1-49867) is a polyclonal antibody validated for use in WB and ELISA. Anti-AF10 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RLEDTTARFTNAN corresponding to internal region according to NP_001009569.1.
• Epitope: RLEDTTARFTNAN - internal region
• Specificity: This antibody is expected to recognize both reported isoforms (NP_004632.1; NP_001009569.1).
• Predicted Species: Human (100%), Mouse (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MLLT10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Experiments gave bands at approx. 140 kDa band and 60 kDa band in rat testis lysates. These bands correspond to earlier findings with different antibodies from other sources. This protein has a calculated MW of 109 kDa band according to human NP_004632.1, 107 kDa band according to human NP_001009569.1and 103 kDa band according to rat NP_001012162.1.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AF10 Antibody

• AF10MGC75086
• ALL1-fused gene from chromosome 10 protein
• DKFZp686E10210
• myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 10
• myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 10
• protein AF-10
• type I AF10 protein

Background

AF10 is a putative transcription factor containing a leucine zipper motif. A t(10;11)(p12;q23) chromosomal translocation present in a proportion of acute myeloid leukaemias results in expression of an AF10 (carboxy terminus) MLL (amino terminus) fusion protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AF10 Antibody (NBP1-49867)(1)

Publication using NBP1-49867

• Lin YH, Kakadia PM, Chen Y et al. Global reduction of the epigenetic H3K79 methylation mark and increased chromosomal instability in CALM-AF10-positive leukemias. Blood 2009-07-01 [PMID: 19443658]

Bioinformatics

• Gene Symbol: MLLT10
• Entrez:
  • 8028(Human)
  • 17354(Mouse)
  • 361285(Rat)
• Uniprot:
  • NP_004632.1(Human)