Adropin Antibody - BSA Free

Western Blot: Adropin Antibody [NBP1-26387] - Total protein from Human and Mouse brain was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-Adropin in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Western Blot: Adropin Antibody [NBP1-26387] - Western blot on Adropin overexpression lysate.

Metabolic molecules in macrophage with adropin treatment. (A) Variations of key molecules involved in glycol–lipid metabolism. (B) Akt, mTOR, and AMPK levels of adropin-treated macrophages. (C) Detection of the above metabolism molecules in ENHO−/− macrophages. (D) Akt, mTOR, and AMPK levels of ENHO−/− macrophages. (E) Effect of ROS depletion or CPT1 alpha inhibition on inflammasome activation. Each experiment was repeated at least twice Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37904094), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Ectopic adropin expression inhibits tumor growth in vivo. (A) Presence of adropin in the indicated cell lines. (B) Ectopic adropin expression in MC38 cells by lentivirus transfection. (C) In vivo tumors formed by MC38 and MC38-ENHO cells. (D) Weights of tumors. Detection of macrophages (E) and CD8+ T cells (F) in tumors. (G) Phenotypic analysis of tumor-infiltrated macrophages. The experiment was repeated twice. ns, no significance; *, P < 0.05; **, P < 0.01; ***, P < 0.001 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37904094), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Correlational analysis of adropin level with local macrophages in CRC. Correlation study of adropin with CD68, ARG1, or iNOS in tumor foci (A) or stromal cells (B). (C) Comparison of infiltrated macrophages between high and low ENHO transcriptions. (D) Correlation study of GPR19 with adropin in foci and stroma. (E) Correlation study of GPR19 with CD68, ARG1, or iNOS. Double staining of adropin and CD68, ARG1, iNOS, or PD-L1 (F) and statistics (G). Double staining of GPR19 and CD68, ARG1, iNOS, or PD-L1 (H) and statistics (I). ns, no significance; *, P < 0.05; **, P < 0.01; ***, P < 0.001 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37904094), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Macrophages in ENHO−/− mice. (A) Adropin variations in macrophages after the treatment of LPS/IFN gamma , IL-4, IL-10, or TGF-beta 1. (B) Macrophage subsets in ENHO−/− mice. (C) CD86 and CD206 expression on WT or KO macrophages treated by LPS/IFN gamma or IL-4. (D) Inhibitory effects of WT or KO macrophages on CD8+ T cells. Inflammasome-associated molecules (E) and effector molecules (F) of WT or KO macrophages. The experiment was carried-out three times. ns, no significance; *, P < 0.05; **, P < 0.01; ***, P < 0.001 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37904094), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.11 mg/ml

Adropin Antibody - BSA Free Summary

• Immunogen: Synthetic peptide made to an internal portion of human Adropin (within residues 10-60). [Swiss-Prot# Q6UWT2]
• Localization: secreted.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ENHO
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/ml
• Application Notes: A band can be seen at 15 kDa in Western Blot. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
  Adropin Overexpression Lysate

• Control Peptide:

  Adropin Peptide (NBP1-26387PEP)

Reactivity Notes

88% sequence identity with bovine protein.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1.11 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Adropin Antibody - BSA Free

• Adropin
• C9orf165
• chromosome 9 open reading frame 165
• energy homeostasis associated
• Energy homeostasis-associated protein
• ENHO
• GAAI470
• UNQ470

Background

Adropin (energy homeostasis-associated protein, ENHO) is a peptide hormone encoded by gene ENHO and its unique ORF generates a 76-residue long secretary peptide which is highly conserved in eutherian (placental) mammals. Adropin is abundantly expressed in liver/brain, and in mouse, hepatic expression as well as circulating adropin concentrations exhibits rapid regulation by fasting (inhibition) and refeeding (stimulation), suggesting regulation through metabolic status signals. Mouse obesity models further confirmed that adropin is involved in metabolic homeostasis and cardiovascular function. Diet-induced as well as genetically induced obesity has been found to be associated with decreased hepatic adropin expression and circulating adropin concentrations. Adropin-knockout mice exhibit increased adiposity and fasting triglycerides (TG), hepatic steatosis, insulin resistance, and increased propensity for impaired glucose tolerance with diet-induced obesity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Adropin Antibody (NBP1-26387)(3)

Publications using NBP1-26387

• Jia L, Liao L, Jiang Y et al. Low-dose adropin stimulates inflammasome activation of macrophage via mitochondrial ROS involved in colorectal cancer progression BMC cancer 2023-10-30 [PMID: 37904094] (WB, Human)
• Lee MJ, Zhu J, An JH et al. A transcriptomic analysis of cerebral microvessels reveals the involvement of Notch1 signaling in endothelial mitochondrial-dysfunction-dependent BBB disruption Fluids and barriers of the CNS 2022-08-26 [PMID: 36028880] (WB, Mouse)
  
  Details:
  Proteins from isolated brain microvessels and brain tissue, dilution used 1:500
• Yang W, Liu L, Wei Y et al. Exercise suppresses NLRP3 inflammasome activation in mice with diet-induced NASH: a plausible role of adropin Laboratory investigation; a journal of technical methods and pathology 2020-12-02 [PMID: 33268842] (WB)

Bioinformatics

• Gene Symbol: ENHO
• Entrez:
  • 375704(Human)
• Uniprot:
  • Q6UWT2()