ADHFE1 Antibody - BSA Free

Immunohistochemistry: ADHFE1 Antibody - BSA Free [NBP3-48209] - Immunohistochemistry of paraffin-embedded human liver tissue slide using (ADHFE1 Antibody) at dilution of 1:50

Western Blot: ADHFE1 Antibody - BSA Free [NBP3-48209] - Mouse heart tissue were subjected to SDS PAGE followed by western blot with (ADHFE1 antibody) at dilution of 1:1000

Immunoprecipitation: ADHFE1 Antibody - BSA Free [NBP3-48209] - IP Result of anti-ADHFE1 (IP:, 4ug; Detection: 1:300) with mouse heart tissue lysate 4000ug.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ADHFE1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ADHFE1 Antibody - BSA Free (NBP3-48209) is a polyclonal antibody validated for use in IHC, WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: alcohol dehydrogenase, iron containing, 1. (Uniprot# Q8IWW8)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADHFE1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:20-1:200
  • Immunoprecipitation 1:200-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ADHFE1 Antibody - BSA Free

• ADH8
• alcohol dehydrogenase, iron containing, 1
• EC 1.1.99.24
• Fe-containing alcohol dehydrogenase
• HOTmitochondrial
• MGC48605

Background

ADHFE1, also known as hydroxyacid-oxoacid transhydrogenase, contains a 50 kDa, 45 kDa, 33 kDa, and 27 kDa isoform, and is involved in oxidation in mammalian tissues. Current research on ADHFE1 includes its relation to a variety of disorders and diseases, including alcohol-related neurodevelopmental disorder, pneumonia, patellar tendinitis, hepatoblastoma, cholangiocarcinoma, Pontiac fever, alcoholism, Legionnaires' disease, and intrahepatic cholangiocarcinoma. This protein is linked to pyruvate metabolism, the citric acid (TCA) cycle, and respiratory electron transport, where it interacts with GNAQ and LIPC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ADHFE1