ADAM10 Antibody - BSA Free

Western Blot: ADAM10 Antibody [NBP2-15280] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM10 antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: ADAM10 Antibody [NBP2-15280] - Paraffin-embedded Lung Papillary Adenocarcinoma, using antibody at 1:250 dilution.

Western Blot: ADAM10 Antibody [NBP2-15280] - Sample (30 ug of whole cell lysate) A: THP-1 7.5% SDS PAGE; antibody diluted at 1:1000.

Western Blot: ADAM10 Antibody [NBP2-15280] - Drosophila tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM10 antibody [N2C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.

Boiled and unboiled RT-4 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM10 antibody [N2C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.(WCE: whole cell extract; ME: membrane extract)

Untreated (-) and treated (+) unboiled A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM10 antibody [N2C1], Internal (NBP2-15280) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Dr, Rt, Po, Bv, Ch
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ADAM10 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ADAM10 Antibody - BSA Free (NBP2-15280) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ADAM10. The exact sequence is proprietary.
• Localization: Cell membrane; Endomembrane system
• Predicted Species: Rat (95%), Porcine (98%), Bovine (97%), Chicken (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADAM10
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 84 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (84%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for ADAM10 Antibody - BSA Free

• a disintegrin and metalloprotease domain 10
• a disintegrin and metalloproteinase domain 10
• AD10
• ADAM 10
• ADAM metallopeptidase domain 10
• ADAM10
• CD156c antigen
• CD156c
• CDw156
• disintegrin and metalloproteinase domain-containing protein 10
• EC 3.4.24
• HsT18717
• kuz
• Kuzbanian protein homolog
• Kuzbanian
• MADM
• MADMEC 3.4.24.81
• Mammalian disintegrin-metalloprotease

Background

Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ADAM10
• Uniprot:
  • O14672()