ADAM12 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: ADAM12 Antibody [NB300-889] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 ...read more
Immunohistochemistry-Paraffin: ADAM12 Antibody [NB300-889] - Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: ADAM12 Antibody [NB300-889] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody ...read more
Immunohistochemistry-Paraffin: ADAM12 Antibody [NB300-889] - Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ADAM12 Antibody Summary

Immunogen
Peptide with sequence AARPLPVSPARALC corresponding to N-Terminus according to NP_003465.3, NP_067673.2.
Specificity
This antibody is expected to recognise both the longer membrane-bound form of human ADAM12 (NP_003465.3) and the shorter soluble ADAM12 splice isoform (NP_067673.2). This antibody does not cross-react with other ADAMS.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ADAM12
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10ug/ml
  • Immunocytochemistry/Immunofluorescence 10ug/ml
  • Immunohistochemistry 3.7 ug/ml
  • Immunohistochemistry-Paraffin 3.7 ug/ml
  • Peptide ELISA Detection limit 1:64000
Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB300-889 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23124962).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ADAM12 Antibody

  • a disintegrin and metalloproteinase domain 12 (meltrin alpha)
  • ADAM 12
  • ADAM metallopeptidase domain 12
  • ADAM12
  • disintegrin and metalloproteinase domain-containing protein 12
  • EC 3.4.24
  • EC 3.4.24.58
  • MCMP
  • MCMPMltna
  • Meltrin alpha
  • meltrin-alpha
  • MLTNA
  • MLTNEC 3.4.24.-

Background

This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF949
Species: Hu, Mu
Applications: WB, Flow, IHC, IP, CyTOF-ready, KO
NBP2-50599
Species: Hu
Applications: WB, ELISA, Flow, IHC, IP, CyTOF-ready
NBP2-53210
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-69364
Species: Hu, Mu
Applications: WB, ICC/IF, IHC
MAB935
Species: Hu
Applications: WB, IHC, IP, CyTOF-ready, ICC, ICFlow
AF1031
Species: Hu
Applications: WB, IP, ICC
NB100-56508
Species: Hu, Mu, Rt, Av
Applications: WB, Flow, IHC, IHC-P, CyTOF-ready, Flow-CS
NBP2-67246
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-91258
Species: Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NB300-889
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA

Publications for ADAM12 Antibody (NB300-889)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: IHC.


Filter By Application
IHC
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
All Species

Reviews for ADAM12 Antibody (NB300-889) (0)

There are no reviews for ADAM12 Antibody (NB300-889). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for ADAM12 Antibody (NB300-889) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional ADAM12 Products

Bioinformatics Tool for ADAM12 Antibody (NB300-889)

Discover related pathways, diseases and genes to ADAM12 Antibody (NB300-889). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ADAM12 Antibody (NB300-889)

Discover more about diseases related to ADAM12 Antibody (NB300-889).
 

Pathways for ADAM12 Antibody (NB300-889)

View related products by pathway.

PTMs for ADAM12 Antibody (NB300-889)

Learn more about PTMs related to ADAM12 Antibody (NB300-889).

Blogs on ADAM12

There are no specific blogs for ADAM12, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ADAM12 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol ADAM12
Entrez
Uniprot