ADAM15 Antibody (23G9)

ADAM15 was detected in immersion fixed MCF‑7 human breast cancer cell line using Human ADAM15 Ectodomain Monoclonal Antibody (Catalog # MAB935) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

ADAM15 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 25 µg/mL Human ADAM15 Ectodomain Monoclonal Antibody (Catalog # MAB935) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC, IP, CyTOF-ready, Flow, ICC/IF
• Clone: 23G9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

ADAM15 Antibody (23G9) Summary

• Immunogen: COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant human ADAM15
  Asp207-Thr696
  Accession # Q13444
• Specificity: Detects the ectodomain of recombinant human (rh) ADAM15 in direct ELISAs and Western blots. In direct ELISAs, shows 100% cross‑reactivity with recombinant mouse (rm) ADAM15 but does not cross-react with rhADAM8, 9, 17, 28, rmADAM9, or 10.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ADAM15
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAM15 Antibody (23G9)

• ADAM 15
• ADAM metallopeptidase domain 15
• ADAM15
• disintegrin-like, and cysteine-rich protein 15
• EC 3.4.24
• MDC15
• MDC-15
• Metargidin

Background

ADAM15 belongs to a family of transmembrane proteins that contain disintegrin and metalloprotease domains. Members of this family have been implicated in cell adhesion via integrin binding and shedding of cell surface molecules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ADAM15 Antibody (MAB935)(3)

Publications using MAB935

• Chatterjee V, Beard R, Reynolds J, Haines R, Guo M, Rubin M, Guido J, Wu M, Yuan S MicroRNA-147b regulates vascular endothelial barrier function by targeting ADAM15 expression. PLoS ONE, 2014;9(10):e110286. 2014 [PMID: 25333931] (Flow Cytometry, Human)
• Ungerer C, Doberstein K, Burger C, Hardt K, Boehncke WH, Bohm B, Pfeilschifter J, Dummer R, Mihic-Probst D, Gutwein P ADAM15 expression is downregulated in melanoma metastasis compared to primary melanoma. Biochem. Biophys. Res. Commun., 2010;401(3):363-9. 2010 [PMID: 20851104] (Flow Cytometry, Human)
• Bohm BB, Aigner T, Roy B, Brodie TA, Blobel CP, Burkhardt H Homeostatic effects of the metalloproteinase disintegrin ADAM15 in degenerative cartilage remodeling. Arthritis Rheum., 2005;52(4):1100-9. 2005 [PMID: 15818704] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: ADAM15
• Uniprot:
  • Q13444()