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ADAM15 Antibody (23G9)

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ADAM15 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 25 µg/mL Human ADAM15 Ectodomain Monoclonal Antibody (Catalog # MAB935) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
ADAM15 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 25 µg/mL Human ADAM15 Ectodomain Monoclonal Antibody (Catalog # MAB935) overnight at 4 °C. Tissue was stained ...read more
ADAM15 was detected in immersion fixed MCF‑7 human breast cancer cell line using Human ADAM15 Ectodomain Monoclonal Antibody (Catalog # MAB935) at 10 µg/mL for 3 hours at room temperature. Cells were stained using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP, CyTOF-ready, Flow, ICC/IF
Clone
23G9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC935U-100UG
Alexa Fluor 405
IC935V-100UG
Alexa Fluor 488
IC935G-100UG
Alexa Fluor 594
IC935T-100UG
Alexa Fluor 647
IC935R-100UG
Alexa Fluor 700
IC935N-100UG
Alexa Fluor 750
IC935S-100UG
Phycoerythrin
IC935P
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
IC935P
Carrier Free
MAB935-SP
Carrier Free
MAB935-500
Carrier Free
IC935U-100UG
Carrier Free
IC935N-100UG
Carrier Free
IC935G-100UG
Carrier Free
IC935T-100UG
Carrier Free
IC935S-100UG
Carrier Free
IC935V-100UG
Carrier Free
IC935R-100UG
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ADAM15 Antibody (23G9) Summary

Immunogen
COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant human ADAM15
Asp207-Thr696
Accession # Q13444
Specificity
Detects the ectodomain of recombinant human (rh) ADAM15 in direct ELISAs and Western blots. In direct ELISAs, shows 100% cross‑reactivity with recombinant mouse (rm) ADAM15 but does not cross-react with rhADAM8, 9, 17, 28, rmADAM9, or 10.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ADAM15
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
Publications
Read Publications using
MAB935 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAM15 Antibody (23G9)

  • ADAM 15
  • ADAM metallopeptidase domain 15
  • ADAM15
  • disintegrin-like, and cysteine-rich protein 15
  • EC 3.4.24
  • MDC15
  • MDC-15
  • Metargidin

Background

ADAM15 belongs to a family of transmembrane proteins that contain disintegrin and metalloprotease domains. Members of this family have been implicated in cell adhesion via integrin binding and shedding of cell surface molecules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ADAM15 Antibody
(23G9)
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ADAM15 Antibody (23G9)
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Applications: WB, IHC, IP, CyTOF-ready, Flow, ICC/IF

Publications for ADAM15 Antibody (MAB935)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Flow Cytometry, Western Blot.


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(2)
Western Blot
(1)
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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 IC935U
Alexa Fluor 405 IC935V
Alexa Fluor 488 IC935G
Alexa Fluor 594 IC935T
Alexa Fluor 647 IC935R
Alexa Fluor 700 IC935N
Alexa Fluor 750 IC935S
Phycoerythrin IC935P
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol ADAM15
Uniprot