ADAM10 Antibody

Images

 
Western Blot: ADAM10 Antibody [NBP1-76973] - Western blot analysis of ADAM10 in Jurkat whole cell lysate with ADAM10 antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: ADAM10 Antibody [NBP1-76973] - Immunofluorescence of ADAM10 in K562 cells with ADAM10 antibody at 10 ug/mL.
Immunocytochemistry/ Immunofluorescence: ADAM10 Antibody [NBP1-76973] - K562 cells using ADAM10 antibody at 2 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ADAM10 Antibody Summary

Immunogen
Antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ADAM10. The immunogen is located within the last 50 amino acids of ADAM10.
Predicted Species
Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ADAM10
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
ADAM10 can be used for detection of ADAM10 by western blot at 1:1000 to 1:2000 dilution. An approximately 85 kDa band can be detected, which may represent precursor.
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
ADAM10 Peptide (NBP1-76973PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ADAM10 Antibody

  • a disintegrin and metalloprotease domain 10
  • a disintegrin and metalloproteinase domain 10
  • AD10
  • ADAM 10
  • ADAM metallopeptidase domain 10
  • ADAM10
  • CD156c antigen
  • CD156c
  • CDw156
  • disintegrin and metalloproteinase domain-containing protein 10
  • EC 3.4.24
  • HsT18717
  • kuz
  • Kuzbanian protein homolog
  • Kuzbanian
  • MADM
  • MADMEC 3.4.24.81
  • Mammalian disintegrin-metalloprotease

Background

Proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-alpha, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-alpha, notch and its ligand delta. The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively. ADAM10 mRNA is expressed in a variety of human and bovine tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

930-ADB
Species: Hu
NBP2-13075
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, IHC-FrFl
MAB931
Species: Hu, Mu
Applications: WB, IHC, CyTOF-ready, ICFlow
AF949
Species: Hu, Mu
Applications: WB, Flow, IHC, IP, CyTOF-ready
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NB300-749
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NB100-56605
Species: Av, Bv, Sh
Applications: WB
MAB935
Species: Hu
Applications: WB, IHC, IP, CyTOF-ready, ICC, ICFlow
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-33939
Species: Hu
Applications: WB, IHC, IHC-P
AF1031
Species: Hu
Applications: WB, IP, ICC
AF976
Species: Hu, Pm
Applications: WB, Flow, IHC, CyTOF-ready, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
NBP2-02149
Species: Hu, Mk
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP1-76973
Species: Hu, Mu, Rt, Bv
Applications: WB, ELISA, ICC/IF

Publications for ADAM10 Antibody (NBP1-76973) (0)

There are no publications for ADAM10 Antibody (NBP1-76973).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ADAM10 Antibody (NBP1-76973) (0)

There are no reviews for ADAM10 Antibody (NBP1-76973). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ADAM10 Antibody (NBP1-76973) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional ADAM10 Products

Bioinformatics Tool for ADAM10 Antibody (NBP1-76973)

Discover related pathways, diseases and genes to ADAM10 Antibody (NBP1-76973). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ADAM10 Antibody (NBP1-76973)

Discover more about diseases related to ADAM10 Antibody (NBP1-76973).
 

Pathways for ADAM10 Antibody (NBP1-76973)

View related products by pathway.

PTMs for ADAM10 Antibody (NBP1-76973)

Learn more about PTMs related to ADAM10 Antibody (NBP1-76973).
 

Research Areas for ADAM10 Antibody (NBP1-76973)

Find related products by research area.

Blogs on ADAM10

There are no specific blogs for ADAM10, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ADAM10 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol ADAM10
Entrez
Uniprot