ADAM10 Antibody - BSA Free

Western Blot: ADAM10 Antibody - BSA Free [NBP1-76973] - KO Validation in MEF Cells.Loading: 10 u of lysate Antibodies: ADAM10, 1 u/mL and beta-actin, 1u/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. Detected both precursor ADAM10 (94KD) and mature ADAM10 (68kD).

Western Blot: ADAM10 Antibody - BSA Free [NBP1-76973] - KO Validation in 293 Cells.Loading: 15 u of lysate Antibodies: ADAM10, 2 u/mL and beta-actin, 1u/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. Detected both precursor ADAM10 (94KD) and mature ADAM10 (68kD).

Immunohistochemistry-Frozen: ADAM10 Antibody - BSA Free [NBP1-76973] - ADAM10 (green) stained human skin, counterstained with DAPI. Tissue fixed, permeablized, and blocked with 5% BSA TBS Ca/Azide. Anti-ADAM10 antibody applied at 1:100, before washed and stained with Alexa488-conjugated secondary. Slides mounted with DAPI. Image from verified customer review.

Immunocytochemistry/ Immunofluorescence: ADAM10 Antibody - BSA Free [NBP1-76973] - Figure 8 Immunofluorescence Validation of ADAM10 in K562 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling ADAM10 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunocytochemistry/ Immunofluorescence: ADAM10 Antibody - BSA Free [NBP1-76973] - Figure 9 Immunofluorescence Validation of ADAM10 in Mouse TestisImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse testis labeling ADAM10 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunocytochemistry/ Immunofluorescence: ADAM10 Antibody - BSA Free [NBP1-76973] - Figure 10 Immunofluorescence Validation of ADAM10 in Rat Testis Immunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling ADAM10 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunocytochemistry/Immunofluorescence: ADAM10 Antibody - BSA Free [NBP1-76973] - K562 cells with ADAM10 antibody at 10 ug/mL.

Western Blot: ADAM10 Antibody - BSA Free [NBP1-76973] - Validation in Human Cell Lines.Loading: 15 u of lysate Antibodies: ADAM10, 1 u/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Detected both precursor ADAM10 (94KD) and mature ADAM10 (68kD).

Western Blot: ADAM10 Antibody - BSA Free [NBP1-76973] - Validation in Rat Tissues.Loading: 15 u of lysates per lane.Antibodies: ADAM10, 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Detected both precursor ADAM10 (94KD) and mature ADAM10 (68kD).

Western Blot: ADAM10 Antibody - BSA Free [NBP1-76973] - Validation in Mouse Tissues.Antibodies: ADAM10, 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Detected both precursor ADAM10 (94KD) and mature ADAM10 (68kD).

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ADAM10 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ADAM10 Antibody - BSA Free (NBP1-76973) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-ADAM10 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ADAM10. The immunogen is located within the last 50 amino acids of ADAM10. Amino Acid Squence: PQRQRPRESYQMGHMRR
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADAM10
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry 2 ug/ml
  • Immunohistochemistry-Frozen
  • Western Blot 1 ug/mL
• Application Notes: IHC- Frozen is reported in verified customer review.
• Theoretical MW: 85 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  ADAM10 Peptide (NBP1-76973PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ADAM10 Antibody - BSA Free

• a disintegrin and metalloprotease domain 10
• a disintegrin and metalloproteinase domain 10
• AD10
• ADAM 10
• ADAM metallopeptidase domain 10
• ADAM10
• CD156c antigen
• CD156c
• CDw156
• disintegrin and metalloproteinase domain-containing protein 10
• EC 3.4.24
• HsT18717
• kuz
• Kuzbanian protein homolog
• Kuzbanian
• MADM
• MADMEC 3.4.24.81
• Mammalian disintegrin-metalloprotease

Background

Proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-alpha, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-alpha, notch and its ligand delta. The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively. ADAM10 mRNA is expressed in a variety of human and bovine tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ADAM10 Antibody (NBP1-76973)(2)

Publications using NBP1-76973

• Aung A, Cui A, Maiorino L et al. Low protease activity in B cell follicles promotes retention of intact antigens after immunization Science (New York, N.Y.) 2023-01-27 [PMID: 36701450] (IHC-Fr, Mouse)
• W Xiao, A Pinilla-Ba, J Faulkner, X Song, P Prabhakar, H Qiu, KW Moremen, A Ludwig, PJ Dempsey, P Azadi, L Wang Robo4 is constitutively shed by ADAMs from endothelial cells and the shed Robo4 functions to inhibit Slit3-induced angiogenesis Scientific Reports, 2022-03-14;12(1):4352. 2022-03-14 [PMID: 35288626]

Review for ADAM10 Antibody (NBP1-76973) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Tyler Crowe IHC-Fr Human 03/08/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Frozen human skin sections
• Species: Human
• Lot: 4439-1204

Comments

• Comments: Tissue fixed, permeablized, and blocked with 5% BSA TBS Ca/Azide. Anti-ADAM10 antibody applied at 1:100, before washed and stained with Alexa488-conjugated secondary. Slides mounted with DAPI.

Bioinformatics

• Gene Symbol: ADAM10
• Uniprot:
  • NP_001101(Human)