ADAM10 Antibody (139712) [Unconjugated]

Cre mRNA is present in the blood plasma of Vav-iCre mice and contained in EVs including exosomes.(A) Cre mRNA can be detected in vesicle preparations enriched for exosomes from the blood plasma of Vav-iCre mice by RT-PCR. Each lane represents a result from an individual animal. For detection of Cre mRNA, nested primer PCR was used. The PCR product at 100 bp represents the signal after the second round of amplification. (B) Cre mRNA is localized in the pellet but not in the supernatant after ultracentrifugation of conditioned medium from primary Vav-iCre–positive hematopoietic cells after stimulation by LPS in vitro. Cre mRNA was resistant to RNaseA treatment in all experiments. (C) After treatment with Triton-X to lyse EVs in combination with RNaseA digestion, Cre mRNA is no longer detectable in contrast to RNaseA treatment alone. (D) Vesicular structures between 50 and 100 nm in size were visualized in electron micrographs from Vav-iCre hematopoietic-cell-derived vesicle preparations (scale bar, 50 nm). (E and F) Secreted membrane vesicle subspecies can be separated by density by sucrose gradient ultracentrifugation. Exosomal identity was confirmed by blotting against the specific protein markers ADAM10 and CD9 for all subfractions. (G) Cre protein could not be detected in any of the fractions. Positive controls for all antibodies are shown in boxes to the right. (H) Cre mRNA is present in the exosomal fractions 2–7. The nonexosomal vesicles fractions or apoptotic bodies are characterized by their variability of positive subfractions to complete absence of Cre mRNA. In this experiment, subfractions 9 and 10 are positive, whereas 8, 11, and 12 do not contain any Cre RNA. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24893313), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready
• Clone: 139712
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

ADAM10 Antibody (139712) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ADAM10
  Thr215-Glu673
  Accession # O35598
• Specificity: Detects mouse ADAM10 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) rhADAM10 is observed and no cross-reactivity with rhADAM8, recombinant mouse (rm) ADAM9, rmADAM15, rhBACE, or rhTACE is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: ADAM10
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAM10 Antibody (139712) [Unconjugated]

• a disintegrin and metalloprotease domain 10
• a disintegrin and metalloproteinase domain 10
• AD10
• ADAM 10
• ADAM metallopeptidase domain 10
• ADAM10
• CD156c antigen
• CD156c
• CDw156
• disintegrin and metalloproteinase domain-containing protein 10
• EC 3.4.24
• HsT18717
• kuz
• Kuzbanian protein homolog
• Kuzbanian
• MADM
• MADMEC 3.4.24.81
• Mammalian disintegrin-metalloprotease

Background

ADAM10 (also known as Kuzbanian, mammalian disintegrin metalloprotease, myelin-associated metalloproteinase) is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). Like other membrane-anchored ADAMs, ADAM10 consists of the following domains, pro with a cysteine switch and furin cleavage sequence, catalytic with the zinc-binding site and Met-turn expected for reprolysins, disintegrin-like, cysteine-rich, EGF-like, transmembrane, and cytoplasmic. ADAM10 is highly conserved, with 97% amino acid identity between mouse, rat, bovine and human and 45% identity between mouse and Drosophila. The active enzyme processes notch, notch ligand delta, and amyloid protein precursor at the alpha site, playing an important role in neurogenesis (3, 4). It also processes the 26 kDa membrane-anchored pro-tumor necrosis factor-alpha (TNF-alpha ) to the 17 kDa mature TNF-alpha (5). It cleaves myelin basic protein and type IV collagen (6, 7). ADAM10 is widely expressed in tissues and resides both on the cell surface and in the cell (8, 9).

1. Rooke, J. et al. (1996) Science 273:1227.
2. Pan, D. and Rubin, G.M. (1997) Cell 90:271.
3. Qi, H. et al. (1999) Science 283:91.
4. Lammich, S. et al. (1999) Proc. Natl. Acad. Sci. USA 96:3922.
5. Rosendahl, M.S. et al. (1997) J. Biol. Chem. 272:24588.
6. Chantry, A. et al. (1989) J. Biol. Chem. 264:21603.
7. Millichip, M.I. et al. (1998) Biochem. Biophys. Res. Comm. 245:594.
8. Chantry, A. and Glynn, P. (1990) Biochem. J. 268:245.
9. Fahrenholz, F.S. et al. (2000) Ann. N.Y. Acad. Sci. 920:215.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ADAM10 Antibody (MAB946)(16)

Publications using MAB946

• Murter, BM;Robinson, SC;Banerjee, H;Lau, L;Uche, UU;Szymczak-Workman, AL;Kane, LP; Downregulation of PIK3IP1/TrIP on T cells is controlled by TCR signal strength, PKC and metalloprotease-mediated cleavage The Journal of biological chemistry 2024-10-23 [PMID: 39454954] (Flow Cytometry, Mouse)
• Thimios A. Mitsiadis, Lucia Jimenez-Rojo, Anamaria Balic, Silvio Weber, Paul Saftig, Pierfrancesco Pagella Adam10-dependent Notch signaling establishes dental epithelial cell boundaries required for enamel formation iScience 10/21/2022 [PMID: 36193048]
• N McMillan, GW Kirschen, S Desai, E Xia, SE Tsirka, A Aguirre ADAM10 facilitates rapid neural stem cell cycling and proper positioning within the subventricular zone niche via JAMC/RAP1Gap signaling Neural regeneration research, 2022-11-01;17(11):2472-2483. 2022-11-01 [PMID: 35535899] (IHC, Mouse)
• G Liu, O Abas, AB Strickland, Y Chen, M Shi CXCR6+CD4+ T cells promote mortality during Trypanosoma brucei infection PloS Pathogens, 2021-10-06;17(10):e1009968. 2021-10-06 [PMID: 34614031] (Flow Cytometry, Mouse)
• Emmanuelle Boscher, Claudia Goupil, Serena Petry, Rémi Keraudren, Andréanne Loiselle, Emmanuel Planel et al. MicroRNA-138 Overexpression Alters A beta 42 Levels and Behavior in Wildtype Mice Frontiers in Neuroscience 1/14/2021 [PMID: 33519353]
• Anke Seifert, Stefan Düsterhöft, Justyna Wozniak, Chek Z. Koo, Michael G. Tomlinson, Elisa Nuti et al. The metalloproteinase ADAM10 requires its activity to sustain surface expression Cellular and Molecular Life Sciences 1/1/2021 [PMID: 32372373]
• J Niven, N Madelon, N Page, A Caruso, G Harlé, S Lemeille, CA Seemayer, S Hugues, M Gannagé Macroautophagy in Dendritic Cells Controls the Homeostasis and Stability of Regulatory T Cells Cell Rep, 2019-07-02;28(1):21-29.e6. 2019-07-02 [PMID: 31269441] (Flow Cytometry, Transgenic Mouse)
• Paul Saftig Tetraspanin 3: A central endocytic membrane component regulating the expression of ADAM10, presenilin and the amyloid precursor protein Biochim. Biophys. Acta, 2016-11-03;0(0):. 2016-11-03 [PMID: 27818272] (Flow Cytometry, Mouse)
• An activated form of ADAM10 is tumor selective and regulates cancer stem-like cells and tumor growth J Exp Med, 2016-08-08;0(0):. 8/8/2016 [PMID: 27503072]
• Altmeppen , Hermann, Prox , Johannes, Krasemann , Susanne, Puig , Berta, Kruszewski , Katharin, Dohler , Frank, Bernreuther , Christia, Hoxha , Ana, Linsenmeier , Luise, Sikorska , Beata, Liberski , Pawel P, Bartsch , Udo, Saftig , Paul, Glatzel , Markus The sheddase ADAM10 is a potent modulator of prion disease. Elife, 2015-02-05;4(0):. 2015-02-05 [PMID: 25654651] (ICC, Mouse)
• Accarias S, Lugo-Villarino G, Foucras G, Neyrolles O, Boullier S, Tabouret G Pyroptosis of resident macrophages differentially orchestrates inflammatory responses to Staphylococcus aureus in resistant and susceptible mice. Eur J Immunol, 2015-01-21;45(3):794-806. 2015-01-21 [PMID: 25472006] (Flow Cytometry, Mouse)
• Ridder K, Keller S, Dams M, Rupp A, Schlaudraff J, Turco D, Starmann J, Macas J, Karpova D, Devraj K, Depboylu C, Landfried B, Arnold B, Plate K, Hoglinger G, Sultmann H, Altevogt P, Momma S Extracellular vesicle-mediated transfer of genetic information between the hematopoietic system and the brain in response to inflammation. PLoS Biol, 2014-06-03;12(6):e1001874. 2014-06-03 [PMID: 24893313] (Western Blot, Mouse)
• Padro C, Shawler T, Gormley M, Sanders V Adrenergic regulation of IgE involves modulation of CD23 and ADAM10 expression on exosomes. J Immunol, 2013-10-18;191(11):5383-97. 2013-10-18 [PMID: 24140643] (Western Blot, Mouse)
• Clifford S. Guy, Kate M. Vignali, Jamshid Temirov, Matthew L Bettini, Abigail E. Overacre, Matthew Smeltzer et al. Distinct T cell receptor signaling pathways drive proliferation and cytokine production in T cells Nature Immunology 3/1/2013 [PMID: 23377202]
• Glomski K, Monette S, Manova K, de Strooper B, Saftig P, Blobel CP Deletion of Adam10 in endothelial cells leads to defects in organ-specific vascular structures. Blood, 2011-06-07;118(4):1163-74. 2011-06-07 [PMID: 21652679] (Western Blot, Mouse)
• Gough PJ, Garton KJ, Wille PT, Rychlewski M, Dempsey PJ, Raines EW A disintegrin and metalloproteinase 10-mediated cleavage and shedding regulates the cell surface expression of CXC chemokine ligand 16. J. Immunol., 2004-03-15;172(6):3678-85. 2004-03-15 [PMID: 15004171] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: ADAM10
• Uniprot:
  • O35598()