Mouse splenocytes were stained with Rat Anti-Mouse ADAM10 Ectodomain Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB946F, filled histogram) or isotype control antibody (Catalog # IC006F, open histogram). ...read more
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ADAM10 Thr215-Glu673 Accession # O35598
Detects mouse ADAM10 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) rhADAM10 is observed and no cross-reactivity with rhADAM8, recombinant mouse (rm) ADAM9, rmADAM15, rhBACE, or rhTACE is observed.
Test in a species/application not listed above to receive a full credit towards a future purchase.
12 months from date of receipt, 2 to 8 °C as supplied.
Supplied in a saline solution containing BSA and Sodium Azide.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for ADAM10 Antibody (139712) [Fluorescein]
a disintegrin and metalloprotease domain 10
a disintegrin and metalloproteinase domain 10
ADAM metallopeptidase domain 10
disintegrin and metalloproteinase domain-containing protein 10
Kuzbanian protein homolog
ADAM10 (also known as Kuzbanian, mammalian disintegrin metalloprotease, myelin-associated metalloproteinase) is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). Like other membrane-anchored ADAMs, ADAM10 consists of the following domains, pro with a cysteine switch and furin cleavage sequence, catalytic with the zinc-binding site and Met-turn expected for reprolysins, disintegrin-like, cysteine-rich, EGF-like, transmembrane, and cytoplasmic. ADAM10 is highly conserved, with 97% amino acid identity between mouse, rat, bovine and human and 45% identity between mouse and Drosophila. The active enzyme processes notch, notch ligand delta, and amyloid protein precursor at the alpha site, playing an important role in neurogenesis (3, 4). It also processes the 26 kDa membrane-anchored pro-tumor necrosis factor-alpha (TNF-alpha ) to the 17 kDa mature TNF-alpha (5). It cleaves myelin basic protein and type IV collagen (6, 7). ADAM10 is widely expressed in tissues and resides both on the cell surface and in the cell (8, 9).
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Fahrenholz, F.S. et al. (2000) Ann. N.Y. Acad. Sci. 920:215.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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