ADAM10 Antibody (139712) [Alexa Fluor® 488] Summary
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ADAM10
Accession # O35598
Detects mouse ADAM10 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) rhADAM10 is observed and no cross-reactivity with rhADAM8, recombinant mouse (rm) ADAM9, rmADAM15, rhBACE, or rhTACE is observed.
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Flow Cytometry 0.25-1 ug/10^6 cells
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.
Packaging, Storage & Formulations
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
0.09% Sodium Azide
Please see the vial label for concentration. If unlisted please contact technical services.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for ADAM10 Antibody (139712) [Alexa Fluor® 488]
- a disintegrin and metalloprotease domain 10
- a disintegrin and metalloproteinase domain 10
- ADAM 10
- ADAM metallopeptidase domain 10
- CD156c antigen
- disintegrin and metalloproteinase domain-containing protein 10
- EC 3.4.24
- Kuzbanian protein homolog
- MADMEC 126.96.36.199
- Mammalian disintegrin-metalloprotease
ADAM10 (also known as Kuzbanian, mammalian disintegrin metalloprotease, myelin-associated metalloproteinase) is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). Like other membrane-anchored ADAMs, ADAM10 consists of the following domains, pro with a cysteine switch and furin cleavage sequence, catalytic with the zinc-binding site and Met-turn expected for reprolysins, disintegrin-like, cysteine-rich, EGF-like, transmembrane, and cytoplasmic. ADAM10 is highly conserved, with 97% amino acid identity between mouse, rat, bovine and human and 45% identity between mouse and Drosophila. The active enzyme processes notch, notch ligand delta, and amyloid protein precursor at the alpha site, playing an important role in neurogenesis (3, 4). It also processes the 26 kDa membrane-anchored pro-tumor necrosis factor-alpha (TNF-alpha ) to the 17 kDa mature TNF-alpha (5). It cleaves myelin basic protein and type IV collagen (6, 7). ADAM10 is widely expressed in tissues and resides both on the cell surface and in the cell (8, 9).
- Rooke, J. et al. (1996) Science 273:1227.
- Pan, D. and Rubin, G.M. (1997) Cell 90:271.
- Qi, H. et al. (1999) Science 283:91.
- Lammich, S. et al. (1999) Proc. Natl. Acad. Sci. USA 96:3922.
- Rosendahl, M.S. et al. (1997) J. Biol. Chem. 272:24588.
- Chantry, A. et al. (1989) J. Biol. Chem. 264:21603.
- Millichip, M.I. et al. (1998) Biochem. Biophys. Res. Comm. 245:594.
- Chantry, A. and Glynn, P. (1990) Biochem. J. 268:245.
- Fahrenholz, F.S. et al. (2000) Ann. N.Y. Acad. Sci. 920:215.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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