ACTL6B Antibody

Western Blot: ACTL6B Antibody [NBP3-18635] - Figure 2. Western blot analysis using the antibody directed against ACTL6B Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using the antibody against ACTL6B diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 47 kDa) is indicated on the right; the marker (in kDa) is shown on the left.

ELISA: ACTL6B Antibody [NBP3-18635] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against ACTL6B . The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:76,000

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ACTL6B Antibody Summary

• Immunogen: Polyclonal antibody raised in rabbit against human ACTL6B (actin-like 6B), using a KLH-conjugated synthetic peptide containing a sequence from the central part of the protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACTL6B
• Purity: Whole Antiserum

Applications/Dilutions

• Dilutions:
  • ELISA 1:500-1:1000
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20-1:50
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antiserum
• Preservative: 0.05% Sodium Azide
• Purity: Whole Antiserum

Alternate Names for ACTL6B Antibody

• 53 kDa BRG1-associated factor B
• actin-like 6
• actin-like 6B
• Actin-related protein Baf53b
• ACTL6
• arpNalpha
• BAF53Bactin-like protein 6B
• BRG1-associated factor 53B
• hArpN alpha

Background

The protein encoded by the ACTL6B gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. This subunit may be involved in the regulation of genes by structural modulation of their chromatin, specifically in the brain. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACTL6B
• Uniprot:
  • O94805(Human)