Activin A Antibody - beta A subunit

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Recombinant Human/Mouse/Rat Activin A (Catalog # 338-AC) increases hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the ...read more
Activin A was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 5 µg/mL Goat Anti-Human/Mouse/Rat Activin A beta A Subunit Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Activin A Antibody - beta A subunit Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Activin A
Specificity
Detects human, mouse and rat Activin A in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
INHBA
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 2-6 ug/mL
Publications
Read Publications using
AF338 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Activin A Antibody - beta A subunit

  • Activin A

Background

Activin and Inhibin are members of the TGF-beta superfamily of cytokines and are involved in a wide range of biological processes including tissue morphogenesis and repair, fibrosis, inflammation, neural development, hematopoiesis, reproductive system function, and carcinogenesis (1‑7). Activin and Inhibin are produced as precursor proteins. Their amino terminal propeptides are proteolytically cleaved and facilitate formation of disulfide-linked dimers of the bioactive proteins (8, 9). Activins are nonglycosylated homodimers or heterodimers of various beta subunits ( beta A, beta B, beta C, and beta E in mammals), while Inhibins are heterodimers of a unique alpha subunit and one of the beta subunits. Activin A is a widely expressed homodimer of two beta A chains. The beta A subunit can also heterodimerize with a beta B or beta C subunit to form Activin AB and Activin AC, respectively (10). The 14 kDa mature human beta A chain shares 100% amino acid sequence identity with bovine, feline, mouse, porcine, and rat beta A. Activin A exerts its biological activities by binding to the type 2 serine/threonine kinase Activin RIIA which then noncovalently associates with the type 1 serine/threonine kinase Activin RIB/ALK-4 (7, 11). Signaling through this receptor complex leads to Smad activation and regulation of activin-responsive gene transcription (7, 11). The bioactivity of Activin A is regulated by a variety of mechanisms (11). BAMBI, Betaglycan, and Cripto are cell-associated molecules that function as decoy receptors or limit the ability of Activin A to induce receptor complex assembly (12‑14). The intracellular formation of Activin A can be prevented by the incorporation of the beta A subunit into Activin AC or Inhibin A (3, 10). And the bioavailability of Activin A is restricted by its incorporation into inactive complexes with alpha 2-Macroglobulin, Follistatin, and FLRG (15, 16).
  1. Kumanov, P. et al. (2005) Reprod. Biomed. Online 10:786.
  2. Maeshima, A. et al. (2008) Endocr. J. 55:1.
  3. Rodgarkia-Dara, C. et al. (2006) Mutat. Res. 613:123.
  4. Werner, S. and C. Alzheimer (2006) Cytokine Growth Factor Rev. 17:157.
  5. Xu, P. and A.K. Hall (2006) Dev. Biol. 299:303.
  6. Shav-Tal, Y. and D. Zipori (2002) Stem Cells 20:493.
  7. Chen, Y.G. et al. (2006) Exp. Biol. Med. 231:534.
  8. Gray, A.M. and A.J. Mason (1990) Science 247:1328.
  9. Mason, A.J. et al. (1996) Mol. Endocrinol. 10:1055.
  10. Thompson, T.B. et al. (2004) Mol. Cell. Endocrinol. 225:9.
  11. Harrison, C.A. et al. (2005) Trends Endocrinol. Metab. 16:73.
  12. Onichtchouk, D. et al. (1999) Nature 401:480.
  13. Gray, P.C. et al. (2002) Mol. Cell. Endocrinol. 188:254.
  14. Kelber, J.A. et al. (2008) J. Biol. Chem. 283:4490.
  15. Phillips, D.J. et al. (1997) J. Endocrinol. 155:65.
  16. Schneyer, A. et al. (2003) Endocrinology 144:1671.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Activin A Antibody (AF338)(14)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Canine, Primate - Cholrocebus pygerythrus (Vervet Monkey).

We have publications tested in 6 applications: ELISA Development, ICC, IHC Frozen, IHC Paraffin-embedded, Neut, WB.


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ELISA Development
(2)
ICC
(1)
IHC Frozen
(1)
IHC Paraffin-embedded
(3)
Neut
(3)
WB
(4)
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Human
(5)
Mouse
(4)
Rat
(3)
Canine
(1)
Primate - Cholrocebus pygerythrus (Vervet Monkey)
(1)
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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using AF338 Applications Species
J Hu, X Wang, YH Tang, YG Shan, Q Zou, ZQ Wang, CX Huang Activin A inhibition attenuates sympathetic neural remodeling following myocardial infarction in rats Mol Med Rep, 2018;17(4):5074-5080. 2018 [PMID: 29393433] (WB,, Rat) WB Rat
C Wallner, H Jaurich, JM Wagner, M Becerikli, K Harati, M Dadras, M Lehnhardt, B Behr Inhibition of GDF8 (Myostatin) accelerates bone regeneration in diabetes mellitus type 2 Sci Rep, 2017;7(1):9878. 2017 [PMID: 28852138] (Neut,, Mouse) Neut Mouse
S Merfeld-Cl, H Lu, X Wu, KL March, DO Traktuev Hypoxia-induced activin A diminishes endothelial cell vasculogenic activity J. Cell. Mol. Med., 2017;0(0):. 2017 [PMID: 28834227] (Neut,, Human) Neut Human
Yang Y, Zhang N, Crombruggen K, Lan F, Hu G, Hong S, Bachert C Differential Expression and Release of Activin A and Follistatin in Chronic Rhinosinusitis with and without Nasal Polyps. PLoS ONE, 2015;10(6):e0128564. 2015 [PMID: 26030615] (IHC Frozen,, Human) IHC Frozen Human
Dussiot M, Maciel T, Fricot A, Chartier C, Negre O, Veiga J, Grapton D, Paubelle E, Payen E, Beuzard Y, Leboulch P, Ribeil J, Arlet J, Cote F, Courtois G, Ginzburg Y, Daniel T, Chopra R, Sung V, Hermine O, Moura I An activin receptor IIA ligand trap corrects ineffective erythropoiesis in beta-thalassemia. Nat Med, 2014;20(4):398-407. 2014 [PMID: 24658077] (ELISA Development,, Human) ELISA Development Human
Yaden B, Wang Y, Wilson J, Culver A, Milner A, Datta-Mannan A, Shetler P, Croy J, Dai G, Krishnan V Inhibition of activin A ameliorates skeletal muscle injury and rescues contractile properties by inducing efficient remodeling in female mice. Am J Pathol, 2014;184(4):1152-66. 2014 [PMID: 24655377] (ELISA Development,, Mouse) ELISA Development Mouse
Chen L, Zhang W, Liang H, Zhou Q, Ding Z, Yang H, Liu W, Wu Y, Man Q, Zhang B, Chen X Activin A induces growth arrest through a SMAD- dependent pathway in hepatic progenitor cells. Cell Commun Signal, 2014;12(0):18. 2014 [PMID: 24628936] (IHC Paraffin-embedded,, Rat) IHC Paraffin-embedded Rat
Seeger P, Bosisio D, Parolini S, Badolato R, Gismondi A, Santoni A, Sozzani S Activin A as a mediator of NK-dendritic cell functional interactions. J Immunol, 2014;192(3):1241-8. 2014 [PMID: 24395917] (Neut,, Human) Neut Human
Zucchini S, Buzzi A, Barbieri M, Rodi D, Paradiso B, Binaschi A, Coffin JD, Marzola A, Cifelli P, Belluzzi O, Simonato M Fgf-2 overexpression increases excitability and seizure susceptibility but decreases seizure-induced cell loss. J. Neurosci., 2008;28(49):13112-24. 2008 [PMID: 19052202] (IHC Paraffin-embedded,, Mouse) IHC Paraffin-embedded Mouse
Lin J, Patel SR, Wang M, Dressler GR The cysteine-rich domain protein KCP is a suppressor of transforming growth factor beta/activin signaling in renal epithelia. Mol. Cell. Biol., 2006;26(12):4577-85. 2006 [PMID: 16738323] (WB,, Mouse) WB Mouse
Show All 14 Publications.

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Product General Protocols

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Blogs on Activin A.

Transforming Growth Factor beta Signaling in Stem Cells
Transforming growth factor beta (TGF beta) signaling along with its family members have been implicated in development and maintenance of various organs. Stem cells are important contributors to this process and are characterized by their ability to s...  Read full blog post.

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Bioinformatics

Gene Symbol INHBA