Actin Gamma 1 Antibody (NH3) [PE]

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-64792PE
    • Availability
      Product Discontinued

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Actin Gamma 1 Antibody (NH3) [PE] Summary

Immunogen
Human monocytes and U937 cell line
Specificity
Recognizes human Filamentous actin (F-actin). The binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the stains the cytoplasm of macrophages strongly, and gives granular, localized nuclear staining of all cell types.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
ACTG1
Purity
IgM purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
IgM purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Actin Gamma 1 Antibody (NH3) [PE]

  • ACT
  • ACTB
  • ACTG
  • actin, cytoplasmic 2
  • actin, gamma 1
  • cytoskeletal gamma-actin
  • deafness, autosomal dominant 20; deafness, autosomal dominant 26
  • DFNA20
  • DFNA26
  • Gamma-actin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Actin Gamma 1 Antibody (NB100-64792PE). (Showing 1 - 1 of 1 FAQ).

  1. What is the recommended IgM isotype (reactivity needs to be Human)? Is there a protocol for F-actin staining using this antibody for flow cytometry experiments?
    • Our F-actin antibody with catalog number NB100-64792PE is a mouse monoclonal with the IgM isotype. We have three mouse IgM isotype controls. Unfortunately none of these are conjugated to PE. When using a directly labeled primary antibody it is important to select an isotype control conjugated to the same fluorochrome or label as the test antibody. Novus offers a custom conjugation service and would be able to PE-conjugate our unlabeled mouse IgM isotype control. If you would like us to do this, please contact Rachel Giesler. She will be able to provide a quote. Here is a link to our standard Flow Cytometry protocol. We do not, unfortunately, have a protocol specifically tailored to NB100-64792PE. Please contact us at technical@novusbio.com if you have any aditional questions.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB100-64792APC
DyLight 350 Labeled NB100-64792UV
DyLight 405 Labeled NB100-64792V
DyLight 488 Labeled NB100-64792G
DyLight 680 Labeled NB100-64792FR
DyLight 755 Labeled NB100-64792IR

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Bioinformatics

Gene Symbol ACTG1
Entrez
Uniprot