Actin Gamma 1 Antibody (NH3) [Alexa Fluor® 488]

Product Discontinued

Actin Gamma 1 Antibody (NH3) [Alexa Fluor® 488] Summary

• Immunogen: Human monocytes and U937 cell line
• Specificity: Recognizes human Filamentous actin (F-actin). The binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the stains the cytoplasm of macrophages strongly, and gives granular, localized nuclear staining of all cell types.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTG1
• Purity: IgM purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Western Blot

Reactivity Notes

Predicted cross-reactivities: Rat, Rabbit, Mouse

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50 mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: IgM purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Actin Gamma 1 Antibody (NH3) [Alexa Fluor® 488]

• ACT
• ACTB
• ACTG
• actin, cytoplasmic 2
• actin, gamma 1
• cytoskeletal gamma-actin
• deafness, autosomal dominant 20; deafness, autosomal dominant 26
• DFNA20
• DFNA26
• Gamma-actin

Background

Actin, gamma 1 (ACTG1) is a member of the actin protein family, a highly conserved protein group with three main isoforms: alpha, beta and gamma. Alpha actins are mainly expressed in muscle tissues where they are a major constituent of the contractile apparatus, whereas beta and gamma actin proteins are widely expressed in most cell types where they are predominantly involved in cell motility and cytoskeleton maintenance. Actin gamma 1 is a cytoplasmic actin isoform usually found in nonmuscle cells.

Actin Gamma 1 antibodies serve as excellent loading controls, due to the proteins' near ubiquitous expressn and strong conservation across most species. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Actin Gamma 1 antibody serves as an excellent loading control.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Actin Gamma 1 Antibody (NB100-64792AF488) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: James Xiao ICC Mouse 09/08/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Primary mouse lung endothelial cells
• Species: Mouse
• Lot: 0915-082917

Comments

• Comments: The primary was diluted in a PBS containing 1%BSA. Cells were permeabilized with PBS containing 0.1% Triton X-100.

Bioinformatics

• Gene Symbol: ACTG1
• Entrez:
  • 71(Human)
• Uniprot:
  • P63261(Human)