Actin Gamma 1 Antibody (2A3) [DyLight 405]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ch, Ma, Rb, ZeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr
Clone
2A3
Clonality
Monoclonal
Host
Mouse

Actin Gamma 1 Antibody (2A3) [DyLight 405] Summary

Immunogen
Peptide comprising the N-terminal nonapeptide of gamma-cytoplasmic actin with an acetylated N-terminus (Ac-EEEIAALVI-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue.
Specificity
The monoclonal antibody Anti-g-CYA, clone 2A3 is an antibody against gamma cytoplasmic actin, the antibody is highly specific for gamma-cytoplasmic actin and does not cross react with other actin isoforms.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ACTG1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
DyLight 405
Format
DyLight 405 (A=400, E=420)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
Application Notes
Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:500 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.

Reactivity Notes

The epitope recognized is highly conserved. The antibody therefore cross-reacts with many species including protochordates, lower craniates and mammals. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Alternate Names for Actin Gamma 1 Antibody (2A3) [DyLight 405]

  • ACT
  • ACTB
  • ACTG
  • actin, cytoplasmic 2
  • actin, gamma 1
  • cytoskeletal gamma-actin
  • deafness, autosomal dominant 20; deafness, autosomal dominant 26
  • DFNA20
  • DFNA26
  • Gamma-actin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Actin Gamma 1 Antibody (NBP1-97720V) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NBP1-97720APC
DyLight 350 Labeled NBP1-97720UV
DyLight 405LS Labeled NBP1-97720V3
DyLight 488 Labeled NBP1-97720G
DyLight 680 Labeled NBP1-97720FR
DyLight 755 Labeled NBP1-97720IR
PE Labeled NBP1-97720PE
PerCP Labeled NBP1-97720PCP

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Blogs on Actin Gamma 1

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol ACTG1

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