ACOX3 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: ACOX3 Antibody [NBP1-85901] - Staining of human cell line A-431 shows localization to peroxisomes. Antibody staining is shown in green.

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ACOX3 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: YRGGYFSGEQAGEVLESAVLALCSQLKDDAVALVDVIAPPDFVLDSPIGRADGELYKNLWGAVLQESKVLERASWWPEFSVNKPVIGSLKSKL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACOX3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Western Blot 0.04 - 0.4 ug/ml
• Application Notes: ICC/IF reported in scientific literature (PMID: 25096407). ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  ACOX3 Protein (NBP1-85901PEP)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25096407).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ACOX3 Antibody - BSA Free

• acyl-CoA oxidase 3, pristanoyl
• Branched-chain acyl-CoA oxidase
• BRCACox
• BRCOX
• EC 1.3.3.6
• peroxisomal acyl-coenzyme A oxidase 3
• PRCOX
• pristanoyl
• Pristanoyl-CoA oxidase

Background

ACOX3, also known as Peroxisomal acyl-coenzyme A oxidase 3, consists of a 78 kDa and a shorter 70 kDa isoform, and is involved in the oxidation of 2-methyl-branched fatty acids in CoA-esters. Disease research is currently being conducted with ACOX3 and its relation to prostate cancer, prostatitis, and leukemia. This protein has been shown to have interactions with SMURF2, SLC2A4, ACAA1, ACAA2, and ACAT1 in the PPAR signaling pathway and a variety of metabolic pathways including those of peroxisomal lipids, lipoproteins, and fatty acids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ACOX3 Antibody (NBP1-85901)(2)

Publications using NBP1-85901

• Chen W, Fan H et al. Taraxacum officinale extract ameliorates dextran sodium sulphate-induced colitis by regulating fatty acid degradation and microbial dysbiosis. J Cell Mol Med 2019-01-12 [PMID: 31565850] (IF/IHC, Mouse)
• Biase FH, Cao X, Zhong S. Cell fate inclination within 2-cell and 4-cell mouse embryos revealed by single-cell RNA sequencing. Genome Res 2014-11-01 [PMID: 25096407] (ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: ACOX3