Human Aconitase 2 ELISA Kit (Colorimetric)

ELISA: Human Aconitase 2 ELISA Kit (Colorimetric) [NBP2-66394] - Samples were spiked with high concentrations of Human Aconitase 2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Aconitase 2 ELISA Kit (Colorimetric) [NBP2-66394] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL

Human Aconitase 2 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human ACO-2 in samples. No significant cross-reactivity or interference between human ACO-2 and analogues was observed.
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.75%
• Intra-Assay: CV% < 5.28%
• Spike Recovery: 89-109%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: ACO2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Aconitase 2 ELISA Kit (Colorimetric)

• aconitase 2, mitochondrial
• Aconitase
• aconitate hydratase, mitochondrial
• ACONM
• Citrate hydro-lyase
• EC 4.2.1
• EC 4.2.1.3
• MGC20605
• MGC33908

Background

Aconitase 2 is encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Aconitase 2 ELISA Kit (NBP2-66394). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ACO2