ACK1 Antibody

Western Blot: ACK1 Antibody [NBP1-32311] - Sample(30 ug of whole cell lysate)A:Raji 7. 5% SDS PAGE, antibody diluted at 1:500.

Immunohistochemistry-Paraffin: ACK1 Antibody [NBP1-32311] - Human lung papillory adenocarcinoma. ACK1 antibody [N1N2], N-term dilution: 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: ACK1 Antibody [NBP1-32311] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.

Product Discontinued

ACK1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human ACK1. The exact sequence is proprietary.
• Localization: Cell membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TNK2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 115 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris-Glycine (pH7), 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for ACK1 Antibody

• ACK1
• ACK-1
• ACK1ACKp21cdc42Hs
• activated CDC42 kinase 1
• activated Cdc42-associated kinase 1
• activated p21cdc42Hs kinase
• EC 2.7.10
• EC 2.7.10.2
• FLJ44758
• FLJ45547
• TNK2
• Tyrosine kinase non-receptor protein 2
• tyrosine kinase, non-receptor, 2

Background

This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: TNK2