Acetyl-CoA Carboxylase alpha/ACACA Antibody (EP687Y)

Product Discontinued

Acetyl-CoA Carboxylase alpha/ACACA Antibody (EP687Y) Summary

• Immunogen: A synthetic peptide corresponding to residues in the human Acetyl-CoA carboxylase 1 was used as an immunogen.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ACACA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-100
  • Immunohistochemistry-Paraffin 1:50-100
  • Western Blot 1:500-2000
• Theoretical MW: 266 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse, Rat. For Mouse and Rat this antibody is recommended for Western Blot only.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Protein A or G purified

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for Acetyl-CoA Carboxylase alpha/ACACA Antibody (EP687Y)

• ACACA
• ACACacetyl-CoA carboxylase 1
• ACC1
• ACC1ACC
• ACCA
• ACC-alpha
• AcetylCoA Carboxylase alpha
• Acetyl-CoA Carboxylase alpha
• acetyl-Coenzyme A carboxylase alpha
• EC 6.4.1.2

Background

Acetyl-CoA carboxylase 1 (ACC1) is a biotin dependent lipogenic enzyme that is highly expressed during adipogenesis. ACC1 catalyzes acetyl-CoA carboxylation, producing malonyl-CoA, a metabolite involved in energy homeostasis regulation. Malonyl-CoA is a two carbon donor in the synthesis of long-chain fatty acids and the elongation of fatty acids found in the cystol (1). ACC1 is regulated short-term by citrate, CoA, and palmitoyl-CoA through allosteric interactions. Nutrients and hormones can be both short-term (inducing reversible phosphorylations by such as AMPK) and long-term (transcription level regulation) regulators of ACC1 (2). Highly expressed in lipogenic tissues, ACC1 is found in liver, adipose, and lactating mammary gland (3). ACC1 has been implicated as a target in the development of anti-obesity drugs (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Acetyl-CoA Carboxylase alpha/ACACA Antibody (NB110-55440)(6)

Publications using NB110-55440

• Ota S, Horigome K, Ishii T et al. Metformin suppresses glucose-6-phosphatase expression by a complex I inhibition and AMPK activation-independent mechanism. Biochem Biophys Res Commun 2009-10-01 [PMID: 19664596] (WB)
• Kim WY, Chang DJ, Hennessy B et al. A Novel Derivative of the Natural Agent Deguelin for Cancer Chemoprevention and Therapy. Cancer Prev Res 1 577-587. 2008-01-01 [PMID: 19139008] (WB)
• Nery FC, Zeng J, NilBP et al. TorsinA binds the KASH domain of nesprins and participates in linkage between nuclear envelope and cytoskeleton. J Cell Sci 2008-10-01 [PMID: 18827015] (WB)
• Wong GW, Krawczyk SA, Kitidis-Mitrokostas C et al. Identification and characterization of CTRP9, a novel secreted glycoprotein, from adipose tissue that reduces serum glucose in mice and forms heterotrimers with adiponectin. FASEB J 23 ) 241-258. 2009-01-01 [PMID: 18787108] (WB)
• Pierre S, Maeurer C, Coste O et al. Toponomics analysis of functional interactions of the ubiquitin ligase PAM (Protein Associated with Myc) during spinal nociceptive processing. Mol Cell Proteomics 2008-12-01 [PMID: 18753128]
• Yi F, Chen QZ, Jin S et al. Mechanism of homocysteine-induced Rac1/NADPH oxidase activation in mesangial cells: role of guanine nucleotide exchange factor Vav2. Cell Physiol Biochem 2007-01-01 [PMID: 17982273]

Bioinformatics

• Gene Symbol: ACACA
• Entrez:
  • 31(Human)
  • 107476(Mouse)
  • 60581(Rat)
• Uniprot:
  • Q13085()