ACE/CD143 Overexpression Lysate

Product Discontinued

ACE/CD143 Overexpression Lysate Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC205392 and based on accession number NM_152831. The protein contains a C-terminal DDK tag.
• Epitope: C-terminal Myc/DDK
• Specificity: Homo sapiens angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 (ACE), transcript variant 3, mRNA.
• Gene: ACE

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ACE/CD143 Overexpression Lysate

• ACE
• ACE1angiotensin converting enzyme, somatic isoform
• angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
• carboxycathepsin
• CD143 antigen
• CD143
• DCP
• DCP1
• DCP1angiotensin-converting enzyme
• dipeptidyl carboxypeptidase 1
• Dipeptidyl carboxypeptidase I
• EC 3.2.1.-
• EC 3.4.15.1
• Kininase II
• MGC26566
• MVCD3
• peptidase P
• testicular ECA

Background

This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Two most abundant alternatively spliced variants of this gene encode two isozymes - the somatic form and the testicular form that are equally active. Multiple additional alternatively spliced variants have been identified but their full length nature has not been determined. Transcript Variant: This variant (3) differs in the 5' and 3' UTR and includes an alternate in-frame exon in the 5' coding region, compared to variant 1. It also uses an alternate splice site in the 3' coding region, compared to variant 1, that results in a frameshift. Isoform (3) is shorter and has a distinct N- and C-terminii, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: ACE