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ACAD9 Antibody

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Western Blot: ACAD9 Antibody [NBP2-92680] - Analysis of extracts of various cell lines, using ACAD9 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Western Blot: ACAD9 Antibody [NBP2-92680] - Analysis of extracts of various cell lines, using ACAD9 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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ACAD9 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ACAD9 (NP_054768.2).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACAD9
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Western Blot 1:500-1:2000
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for ACAD9 Antibody

  • ACAD-9
  • acyl-CoA dehydrogenase family member 9, mitochondrial
  • acyl-CoA dehydrogenase family, member 9
  • acyl-Coenzyme A dehydrogenase family, member 9
  • EC 1.3.99
  • EC 1.3.99.-
  • EC 1.3.99.10
  • FLJ23533
  • MGC14452
  • NPD002

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-89290
Immunocytochemistry/Immunofluorescence: ACADS Antibody [NBP1-89290] - Staining of human cell line Hep G2 shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ACADS Antibody [NBP1-89290] - Staining of human tonsil shows granular cytoplasmic positivity in germinal center cells.
Immunohistochemistry-Paraffin: ACADS Antibody [NBP1-89290] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
ACADVL Antibody
NBP2-15238
Immunohistochemistry-Paraffin: ACADVL Antibody [NBP2-15238] - Human A549 xenograft. ACADVL antibody [N1C1] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Western Blot: ACADVL Antibody [NBP2-15238] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACADVL antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: ACADVL Antibody [NBP2-15238] - ACADVL antibody detects ACADVL protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ACADVL stained by ACADVL antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
ACADM Antibody
(JE48-09)
NBP2-80447
Western Blot: ACADM Antibody (JE48-09) [NBP2-80447] - Analysis of ACADM on mouse liver tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/Immunofluorescence: ACADM Antibody (JE48-09) [NBP2-80447] - ICC staining of ACADM in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1:50) for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:100 dilution. The nuclear counter stain is DAPI (blue).
Immunocytochemistry/Immunofluorescence: ACADM Antibody (JE48-09) [NBP2-80447] - ICC staining of ACADM in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1:100) for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:100 dilution. The nuclear counter stain is DAPI (blue).
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
CPT1A Antibody
NB100-53791
Western Blot: CPT1A Antibody [NB100-53791] - Staining of MCF7 cell lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence.
(8 Publications)
Species: Hu, Mu, Rt
Applications: PEP-ELISA, WB
ACAD10 Antibody
NBP2-49511
Immunohistochemistry-Paraffin: ACAD10 Antibody [NBP2-49511] - Staining of human lymph node shows moderate cytoplasmic positivity in cells in non-germinal center cells.
Western Blot: ACAD10 Antibody [NBP2-49511] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MG
Species: Hu
Applications: IHC, IHC-P, WB
ETFDH Antibody
NBP1-83950
Western Blot: ETFDH Antibody [NBP1-83950] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
Immunohistochemistry-Paraffin: ETFDH Antibody [NBP1-83950] - Staining of human duodenum shows high expression.
Immunohistochemistry-Paraffin: ETFDH Antibody [NBP1-83950] - Staining of human pancreas shows low expression as expected.
Species: Hu
Applications: IHC, IHC-P, WB
ACAD11 Antibody
NBP2-14255
Immunohistochemistry-Paraffin: ACAD11 Antibody [NBP2-14255] - Staining in human kidney and pancreas tissues.. Corresponding ACAD11 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ACAD11 Antibody [NBP2-14255] - Staining of human skeletal muscle shows moderate granular cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: ACAD11 Antibody [NBP2-14255] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Species: Hu
Applications: IHC, IHC-P
ECSIT Antibody
NBP1-91858
Western Blot: ECSIT Antibody [NBP1-91858] - Analysis in human cell lines PC-3 and HeLa using Anti-ECSIT antibody. Corresponding ECSIT RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Immunocytochemistry/Immunofluorescence: ECSIT Antibody [NBP1-91858] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ECSIT Antibody [NBP1-91858] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB

M-Cadherin/Cadherin-15 ...
AF4096
M‑Cadherin/Cadherin-15 was detected in immersion fixed C2C12 mouse myoblast cell line using Sheep Anti-Human M‑Cadherin/Cadherin-15 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4096) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern­Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter­stained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
C2C12 mouse myoblast cell line was stained with Sheep Anti-Human M‑Cadherin/Cadherin‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4096, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human M-Cadherin/Cadherin-15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4096) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for M-Cadherin/Cadherin-15 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
(2 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, ICC, WB
NDUFAF1 Antibody
(JE47-18)
NBP2-80468
Immunocytochemistry/Immunofluorescence: NDUFAF1 Antibody (JE47-18) [NBP2-80468] - ICC staining NDUFAF1 in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with NDUFAF1 antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Immunocytochemistry/Immunofluorescence: NDUFAF1 Antibody (JE47-18) [NBP2-80468] - ICC staining NDUFAF1 in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with NDUFAF1 antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:100 dilution. The nuclear counter stain is DAPI (blue).
Immunohistochemistry-Paraffin: NDUFAF1 Antibody (JE47-18) [NBP2-80468] - Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-NDUFAF1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1:200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
IVD Antibody
(OTI1B10)
NBP2-03047
Western Blot: IVD Antibody (1B10) [NBP2-03047] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IVD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IVD.
Immunohistochemistry-Paraffin: IVD Antibody (1B10) [NBP2-03047] - Staining of paraffin-embedded Human lymph node tissue using anti-IVD mouse monoclonal antibody.
Immunocytochemistry/Immunofluorescence: IVD Antibody (1B10) [NBP2-03047] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IVD.
Species: Ca, Hu, Pm, Mu, Pm, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
CHPT1 Antibody
NBP2-92299
Western Blot: CHPT1 Antibody [NBP2-92299] - Analysis of extracts of DU145 cells, using CHPT1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.
(1 Review)
Species: Hu
Applications: WB
GCDH Antibody
NBP2-48549
Immunohistochemistry-Paraffin: GCDH Antibody [NBP2-48549] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: GCDH Antibody [NBP2-48549] - Staining of human pancreas shows moderate granular cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: GCDH Antibody [NBP2-48549] - Staining of human testis shows weak granular cytoplasmic positivity.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
BRCA2 Antibody
(234403) [Unconjugate
MAB2476
Western blot shows lysates of Capan‑1 human pancreatic adenocarcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and U2OS human osteosarcoma cell line. PVDF membrane was probed with 0.2 µg/mL of Human BRCA2 Monoclonal Antibody (Catalog # MAB2476) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for BRCA2 at approximately 380 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(6 Publications)
Species: Hu
Applications: IHC, WB
Caldesmon/CALD1
Antibody
NBP1-85702
Western Blot: Caldesmon/CALD1 Antibody [NBP1-85702] - Analysis in human cell lines U-251MG and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody [NBP1-85702] - Staining in human smooth muscle and skeletal muscle tissues using anti-CALD1 antibody. Corresponding CALD1 RNA-seq data are presented for the same tissues.
Immunocytochemistry/Immunofluorescence: Caldesmon/CALD1 Antibody [NBP1-85702] - Staining of human cell line U-2 OS shows localization to plasma membrane and actin filaments. Antibody staining is shown in green.
(4 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
COQ2 Antibody
NBP2-56780
Western Blot: COQ2 Antibody [NBP2-56780] - Analysis NBP2-56780 (A) shows similar pattern to independent antibody NBP2-49563 (B).
Immunocytochemistry/Immunofluorescence: COQ2 Antibody [NBP2-56780] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.
Species: Hu
Applications: ICC/IF, WB
FANCD2 Antibody -
BSA Free
NB100-182
Immunocytochemistry/Immunofluorescence: FANCD2 Antibody [NB100-182] - Analysis using the Biotin conjugate of FANCD2 Antibody. FANCD2 colocalizes in vivo with another protein in SiHa cells after cell exposure to IR. Proliferating SiHa cells were exposed to 10 Gy of IR and double color immunofluorescence staining was performed after 8 h. Images were captured in a Kodak digital image system on a Leica fluorescence microscope.
Knockdown Validated: FANCD2 Antibody [NB100-182] - Resistance of FANCA-mutant cells with defective FANCD2 function to hydrogen peroxide (H2O2). Representative images illustrating staining for subnuclear FANCD2 foci in isogenic fibroblast pairs, either deficient for FANCD2 (PD20) or FANCA (PD220) and their respective wild-type (wt) complemented counterparts. Foci were visualized three hours after treatment with H2O2 (25 uM for 2 hours). Image collected and cropped by CiteAb from the following publication (http://www.hindawi.com/journals/bmri/2008/821529/) licensed under a CC-BY licence.
Western Blot: FANCD2 Antibody [NB100-182] - AICAR-induced FANCD2 monoubiquitination is dependent on AMPK. Inhibitor of AMPK blocks AICAR-induced FANCD2 monoubiquitination in GM00637I normal fibroblasts. Cells were pretreated with 5 um of Compound C (an AMPK inhibitor) 1 h before treatment with 0.25 mm AICAR for 24 h. Cell lysates were subjected to immunoblotting with FANCD2 Antibody to visualize monoubiquitinated FANCD2 (Ub-ANCD2). Image collected and cropped by CiteAb from the following publication (http://doi.wiley.com/10.1002/2211-5463.12185) licensed under a CC-BY licence.
(8 Reviews)
(191 Publications)
Species: Av, Ca, Hu, Ma, Mu, Pm, Rt, Ze
Applications: ChIP, Flow, IB, ICC/IF, IHC, IHC-P, IP, KD, KO, RNAi, Simple Western, WB
ACADL Antibody
NBP1-89289
Immunohistochemistry-Paraffin: ACADL Antibody [NBP1-89289] - Analysis in human thyroid gland and cerebral cortex tissues. Corresponding ACADL RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ACADL Antibody [NBP1-89289] - Staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ACADL Antibody [NBP1-89289] - Staining of human lung shows moderate to strong granular cytoplasmic positivity in macrophages.
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB

Publications for ACAD9 Antibody (NBP2-92680) (0)

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Bioinformatics

Gene Symbol ACAD9