ECSIT Antibody

Images

 
Orthogonal Strategies: Western Blot: ECSIT Antibody [NBP1-91858] - Analysis in human cell lines PC-3 and HeLa using Anti-ECSIT antibody. Corresponding ECSIT RNA-seq data are presented for the same cell lines. ...read more
Immunocytochemistry/ Immunofluorescence: ECSIT Antibody [NBP1-91858] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ECSIT Antibody [NBP1-91858] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
Western Blot: ECSIT Antibody [NBP1-91858] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

ECSIT Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: HMEPDLSARVTIYQVPLPKDSTGAADPPQPHIVGIQSPDQQAALARHNPARPVFVEGPFSLWLRNKCVYYHILRADLLPPEEREVEETPEEWNLYYPM
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ECSIT
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ECSIT Protein (NBP1-91858PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ECSIT Antibody

  • ECSIT homolog (Drosophila)
  • evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial
  • likely ortholog of mouse signaling intermediate in Toll pathway evolutionarilyconserved
  • Protein SITPEC
  • signaling intermediate in Toll pathway evolutionarily conserved ortholog
  • SITPECsignaling intermediate in Toll pathway, evolutionarily conserved

Background

Activation of NF-kB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses (reviewed in 1). Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 (2) to kinases such as IRAK (3) and other intermediates such as TNF receptor associated factor (TRAF)-6 (4). ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway (5). Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis (6), indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional ECSIT Products

Array NBP1-91858

Research Areas for ECSIT Antibody (NBP1-91858)

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Blogs on ECSIT

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Bioinformatics

Gene Symbol ECSIT