ABO, Blood Group A Antigen Antibody (HE-10)

Immunohistochemistry-Paraffin: ABO, Blood Group A Antigen Antibody (HE-10) [NBP2-44633] - Formalin-fixed, paraffin-embedded human Colorectal Carcinoma stained with ABO, Blood Group A Antigen Antibody (HE-10)

• Reactivity: Hu
• Applications: ICC/IF, IHC, IHC-P, Aggl, ICC/IF
• Clone: HE-10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

ABO, Blood Group A Antigen Antibody (HE-10) Summary

• Immunogen: Mixture of erythrocytes of blood group A and glyco protein fraction isolated from the saliva of secretors with blood group A
• Localization: Cell Surface
• Specificity: This monoclonal antibody preferably reacts with determinants of chain A and H type 3(Gal1-3GalNAc-R) and 4 (Gal1-3GalNAc-R), but not with type 1 and 2 chain structures. It is not reactive with immuno-dominant A trisaccharide. This monoclonal antibody is applicable for tissue staining in tumor patients with blood groups A and AB. It shows a highly heterogeneous reactivity in human colon tumor tissue and adjacent mucosa. Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
• Isotype: IgM Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ABO
• Purity: Protein L or PEG purified

Applications/Dilutions

• Dilutions:
  • Agglutination
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein L or PEG purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47871)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for ABO, Blood Group A Antigen Antibody (HE-10)

• A transferase
• A3GALNT
• A3GALT1
• ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase;transferase B, alpha 1-3-galactosyltransferase)
• ABO glycosyltransferase
• B transferase
• B(A) alpha-1,3-galactosyltransferase
• EC 2.4.1.37
• EC 2.4.1.40
• Fucosylglycoprotein 3-alpha-galactosyltransferase
• Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase
• Glycoprotein-fucosylgalactoside alpha-galactosyltransferase
• Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase
• GTB
• Histo-blood group A transferase
• histo-blood group A2 transferase
• histo-blood group ABO system transferase
• Histo-blood group B transferase
• NAGAT

Background

Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ABO
• Uniprot:
  • P16442(Human)