ABCA1 Antibody (1276B) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB
• Clone: 1276B
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 532

ABCA1 Antibody (1276B) [Alexa Fluor® 532] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Human ABCA1 synthetic peptide
  Accession # O95477
• Specificity: Detects human ABCA1 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ABCA1 Antibody (1276B) [Alexa Fluor® 532]

• ABC1
• ABC-1
• ABC1ATP-binding cassette transporter A1
• ABCA1
• ATP-binding cassette 1
• ATP-binding cassette transporter 1
• ATP-binding cassette, sub-family A (ABC1), member 1
• CERP
• Cholesterol efflux regulatory protein
• EC 2.7.7.8
• EC 2.8.1.8
• EC 3.6.3
• EC 3.6.3.41
• FLJ14958
• HDLDT1
• membrane-bound
• MGC164864
• MGC165011
• TGD
• TGDATP-binding cassette sub-family A member 1

Background

ABCA1 (ATP-binding cassette, subfamily A member 1; also cholesterol efflux regulatory protein) is a 240-280 kDa member of the ABCA family, ABC transporter superfamily of molecules. It is widely expressed, particularly by macrophages, where it participates in the transport of lipid across cell membranes. Once transported, it is passed on to tethered apoA-I to form HDL. Human ABCA1 is 2261 amino acids (aa) in length. It has up to 15 transmembrane segments with beginning and ending cytoplasmic domains and a large cytoplasmic central region. ABC transporters are found between aa 899-1311 and 1912-2144. Amino acids 2216-2221 are essential for lipid transport, and two intramolecular disulfide bonds are required for activity (Cys75-Cys309 and Cys1463/1465-Cys1477). There are three potential isoform variants. One possesses an alternative start site at Met61, while two others contain 12 aa and 123 aa substitutions for aa 241-2261, respectively. Over aa 1248-1350, human ABCA1 shares 97% aa identity with mouse ABCA1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for ABCA1 Antibody (FAB72071X). (Showing 1 - 1 of 1 FAQ).

1. For Western blot protocol for ABCA1 antibody NB400-105, why does it suggest treating with beta-ME while not heating?
   • For some membrane proteins it is not recommended to boil the samples. You may incubate them at room temperature or at 37C in SDS sample buffer with BME. However, from personal experience, if you boil the samples you will not see the protein on the blot. I do not think the exact reason is known.