AATF Antibody - BSA Free

Western Blot: AATF Antibody [NBP1-76655] - Analysis of AATF in human small intestine cell lysate using this antibody at (A) 0.5, (B) 1 ug/ml and (C) 2 ug/ml.

Immunohistochemistry: AATF Antibody - BSA Free [NBP1-76655] - Immunohistochemistry of AATF in human small intestine tissue with AATF antibody at 10 ug/mL.

Immunocytochemistry/ Immunofluorescence: AATF Antibody - BSA Free [NBP1-76655] - Immunofluorescence of AATF in Human Small Intestine cells with AATF antibody at 20 ug/mL.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

AATF Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit AATF Antibody - BSA Free (NBP1-76655) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 12 amino acid synthetic peptide from near the carboxy terminus of human AATF. The immunogen is located within the last 50 amino acids of AATF. Amino Acid Squence: GQLHPPDEGHGD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AATF
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 0.5-2 ug/ml
• Control Peptide:

  AATF Peptide (NBP1-76655PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Affinity purified

Alternate Names for AATF Antibody - BSA Free

• apoptosis antagonizing transcription factor
• Apoptosis-antagonizing transcription factor
• CHE-1
• CHE1Rb-binding protein Che-1
• DEDprotein AATF

Background

AATF (apoptosis antagonizing transcription factor) was initially discovered as an interaction partner of ZIP kinase (ZIPK), a member of death-associated protein (DAP) kinase family of pro-apoptotic serine/threonine kinases. AATF is a phosphoprotein containing an acidic region and a putative leucine zipper domain and nuclear localization signal, features which are typical of transcription factors. AATF inhibits the ZIPK-mediated pro-apoptotic pathway and may activate other anti-apoptotic pathways. Recently, it has also been shown to protect neural cells against oxidative damage induced by amyloid b-peptide and to inhibit aberrant production of the beta-peptide by interacting with Par-4 (prostate apoptosis response-4), another pro-apoptotic leucine zipper protein that is associated with neuronal degeneration in Alzheimer's disease (AD), suggesting that AATF may have potential therapeutic applications in both familial and sporadic forms of AD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AATF
• Uniprot:
  • NP_036270(Human)