AATF Antibody

Western Blot: AATF Antibody [NB100-55324] - Detection of Human AATF/Che-1 on HeLa whole cell lysates using NB100-55324. AATF/Che-1 was also immunoprecipitated by rabbit anti-AATF/Che-1 antibody NB100-55325.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

AATF Antibody Summary

• Description: This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-322, NB100-97831, NB600-233
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 125 and 175 of human apoptosis antagonizing transcription factor (Che-1) using the numbering given in entry NP_036270.1 (GeneID 26574).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AATF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Control:

  AATF Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Notes

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-322, NB100-97831, NB600-233

Alternate Names for AATF Antibody

• apoptosis antagonizing transcription factor
• Apoptosis-antagonizing transcription factor
• CHE-1
• CHE1Rb-binding protein Che-1
• DEDprotein AATF

Background

The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AATF Antibody (NB100-55324)(1)

Publication using NB100-55324

• Moore HM, Bai B, Boisvert FM et al. Quantitative Proteomics and Dynamic Imaging of the Nucleolus Reveal Distinct Responses to UV and Ionizing Radiation. Mol Cell Proteomics. 2011-07-21 [PMID: 21778410]

Bioinformatics

• Gene Symbol: AATF
• Entrez:
  • 26574(Human)
  • 56321(Mouse)
• Uniprot:
  • Q9NY61(Human)