AATF Antibody

Images

 
Western Blot: AATF Antibody [NB100-55324] - Detection of Human AATF/Che-1 on HeLa whole cell lysates using NB100-55324. AATF/Che-1 was also immunoprecipitated by rabbit anti-AATF/Che-1 antibody NB100-55325.
Proximity Ligation Assay: AATF Antibody [NB100-55324] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human ATM (NB100-271) and rabbit anti-human AATF (NB100-55324). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: AATF Antibody [NB100-55324] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human CHK1 (NB100-274) and rabbit anti-human AATF (NB100-55324). Image merged from DAPI (2ms) and ...read more
Proximity Ligation Assay: AATF Antibody [NB100-55324] - Secondary-conjugate Duolink In Situ PLA in Hela cells. goat anti-human CHK1 (NB100-311) and rabbit anti-human AATF (NB100-55324). Image merged from DAPI ...read more
Proximity Ligation Assay: AATF Antibody [NB100-55324] - Secondary-conjugate Duolink In Situ PLA in Hela cells. goat anti-human RelA (NBP2-22297) and rabbit anti-human AATF (NB100-55324). Image merged from DAPI ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

       

Order Details

AATF Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 125 and 175 of human apoptosis antagonizing transcription factor (Che-1) using the numbering given in entry NP_036270.1 (GeneID 26574).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AATF
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Proximity Ligation Assay 1:200-1:2000
Positive Control
AATF Lysate (NBL1-07168)
Publications
Read Publication using NB100-55324.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Notes

This anitbody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-322, NB100-97831, NB600-233

Alternate Names for AATF Antibody

  • apoptosis antagonizing transcription factor
  • Apoptosis-antagonizing transcription factor
  • CHE-1
  • CHE1Rb-binding protein Che-1
  • DEDprotein AATF

Background

The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AATF Antibody (NB100-55324)(1)

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Product General Protocols

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AATF
Entrez
Uniprot