AATF Overexpression Lysate (Native)

Images

 
Western Blot: AATF Overexpression Lysate (Adult Normal) [NBL1-07168] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AATF.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

AATF Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200070 and based on accession number NM_012138. The protein contains a C-terminal DDK tag.
Epitope
C-terminal Myc/DDK
Specificity
Homo sapiens apoptosis antagonizing transcription factor (AATF), mRNA.
Gene
AATF

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for AATF

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for AATF Overexpression Lysate (Native)

  • apoptosis antagonizing transcription factor
  • Apoptosis-antagonizing transcription factor
  • CHE-1
  • CHE1Rb-binding protein Che-1
  • DEDprotein AATF

Background

The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for AATF Lysate (NBL1-07168) (0)

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Bioinformatics

Gene Symbol AATF